Macular pigments (MP), composed of lutein, zeaxanthin and meso-zeaxanthin, accumulate in the human fovea and selectively absorb short-wavelength light, potentially influencing spatial vision. This study investigated the relationship between macular pigment optical density (MPOD) and fine spatial resolution across different wavelengths under conditions subject to light scatter. Sixty healthy participants (mean age = 22.7 years) underwent MPOD assessment using heterochromatic flicker photometry and performed a two-point resolution task utilizing a custom optical system with monochromatic and broadband light sources. A significant (p < 0.05) negative correlation was found between MPOD and two-point resolution thresholds at shorter wavelengths but not at longer wavelengths suggesting MP enhances spatial resolution specifically for absorbed wavelengths. Quartile analysis, for example, demonstrated that individuals with higher MPOD exhibited 31–38 % better spatial resolution at 420–540 nm compared to those with lower MPOD. These findings support the hypothesis that MP selectively mitigates light scatter effects and improves visual function even in low-light conditions.