Dzobo K, Thomford NE, Senthebane DA, Shipanga H, Rowe A, Dandara C, et al. Advances in regenerative medicine and tissue engineering: innovation and transformation of medicine. Stem cells international. 2018;2018(1):2495848.

PubMed  PubMed Central  Google Scholar 

Irawan V, Higuchi A, Ikoma T. Physical cues of biomaterials guide stem cell fate of differentiation: the effect of elasticity of cell culture biomaterials. Open Phys. 2018;16(1):943–55.

Article  CAS  Google Scholar 

Xing F, Li L, Zhou C, Long C, Wu L, Lei H, et al. Regulation and directing stem cell fate by tissue engineering functional microenvironments: scaffold physical and chemical cues. Stem cells international. 2019;2019(1):2180925.

PubMed  PubMed Central  Google Scholar 

Huang F, Wang H, Zhang Y, Wei G, Xie Y, Wu G. Synergistic effect of QNZ, an inhibitor of NF-κB signaling, and bone morphogenetic protein 2 on osteogenic differentiation in mesenchymal stem cells through fibroblast-induced yes-associated protein activation. Int J Mol Sci. 2023;24(9): 7707.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chetty SC, Rost MS, Enriquez JR, Schumacher JA, Baltrunaite K, Rossi A, et al. Vegf signaling promotes vascular endothelial differentiation by modulating etv2 expression. Dev Biol. 2017;424(2):147–61.

Article  PubMed Central  Google Scholar 

Olivares-Navarrete R, Lee EM, Smith K, Hyzy SL, Doroudi M, Williams JK, et al. Substrate stiffness controls osteoblastic and chondrocytic differentiation of mesenchymal stem cells without exogenous stimuli. PLoS One. 2017;12(1): e0170312.

Article  PubMed  PubMed Central  Google Scholar 

Chang Y-J, Chen Y-J, Huang C-W, Fan S-C, Huang B-M, Chang W-T, et al. Cyclic stretch facilitates myogenesis in C2C12 myoblasts and rescues thiazolidinedione-inhibited myotube formation. Front Bioeng Biotechnol. 2016;4: 27.

Article  PubMed  PubMed Central  Google Scholar 

Wang Y, Li J, Han H, Huang H, Du H, Cheng L, et al. Application of locally responsive design of biomaterials based on microenvironmental changes in myocardial infarction. iScience. 2023. https://doi.org/10.1016/j.isci.2023.107662.

Article  PubMed  PubMed Central  Google Scholar 

Zhang J, Klos M, Wilson GF, Herman AM, Lian X, Raval KK, et al. Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich method. Circ Res. 2012;111(9):1125–36.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sridhar S, Venugopal JR, Sridhar R, Ramakrishna S. Cardiogenic differentiation of mesenchymal stem cells with gold nanoparticle loaded functionalized nanofibers. Colloids Surf, B. 2015;134:346–54.

Article  CAS  Google Scholar 

Shevchenko K, Zadorin Y, Zadorina O. Differentiation of embryonic stem cells into cardiac myocytes. Vascul Pharmacol. 2006;3(45):e34–5.

Article  Google Scholar 

Zhang X, Zhang S, Wang T. How the mechanical microenvironment of stem cell growth affects their differentiation: a review. Stem Cell Res Ther. 2022;13(1):415.

Article  PubMed  PubMed Central  Google Scholar 

Chen C, Bai X, Ding Y, Lee I-S. Electrical stimulation as a novel tool for regulating cell behavior in tissue engineering. Biomater Res. 2019;23(1):25.

Article  PubMed  PubMed Central  Google Scholar 

Hammerick KE, James AW, Huang Z, Prinz FB, Longaker MT. Pulsed direct current electric fields enhance osteogenesis in adipose-derived stromal cells. Tissue Eng Part A. 2010;16(3):917–31.

Article  CAS  PubMed  Google Scholar 

Thrivikraman G, Boda SK, Basu B. Unraveling the mechanistic effects of electric field stimulation towards directing stem cell fate and function: a tissue engineering perspective. Biomaterials. 2018;150:60–86.

Article  CAS  PubMed  Google Scholar 

Mohammadi Amirabad L, Massumi M, Shamsara M, Shabani I, Amari A, Mossahebi Mohammadi M, et al. Enhanced cardiac differentiation of human cardiovascular disease patient-specific induced pluripotent stem cells by applying unidirectional electrical pulses using aligned electroactive nanofibrous scaffolds. ACS Appl Mater Interfaces. 2017;9(8):6849–64.

Article  CAS  PubMed  Google Scholar 

Tandon N, Cannizzaro C, Chao P-HG, Maidhof R, Marsano A, Au HTH, et al. Electrical stimulation systems for cardiac tissue engineering. Nature protocols. 2009;4(2):155–73.

Tandon N. Biomimetic electrical stimulation for cardiac tissue engineering [dissertation]. Cambridge (MA): Massachusetts Institute of Technology; 2006.

Tandon N, Goh B, Marsano A, Chao P-HG, Montouri-Sorrentino C, Gimble J, et al., editors. Alignment and elongation of human adipose-derived stem cells in response to direct-current electrical stimulation. 2009 annual international conference of the IEEE engineering in medicine and biology society. IEEE. 2009.

Llucià-Valldeperas A, Sanchez B, Soler-Botija C, Gálvez-Montón C, Roura S, Prat-Vidal C, et al. Physiological conditioning by electric field stimulation promotes cardiomyogenic gene expression in human cardiomyocyte progenitor cells. Stem Cell Res Ther. 2014;5:1–5.

Article  Google Scholar 

Qian Q, Qian H, Zhang X, Zhu W, Yan Y, Ye S, et al. 5-azacytidine induces cardiac differentiation of human umbilical cord-derived mesenchymal stem cells by activating extracellular regulated kinase. Stem Cells Dev. 2012;21(1):67–75.

Article  CAS  PubMed  Google Scholar 

Li C, Fan Y, Zheng L. Differentiation of stem cells regulated by biophysical cues. Sheng wu yi xue Gong Cheng xue za zhi= Journal of Biomedical Engineering= Shengwu Yixue Gongchengxue Zazhi. 2023;40(4):609–16.

PubMed  Google Scholar 

Barash Y, Dvir T, Tandeitnik P, Ruvinov E, Guterman H, Cohen S. Electric field stimulation integrated into perfusion bioreactor for cardiac tissue engineering. Tissue Eng Part C Methods. 2010;16(6):1417–26.

Article  CAS  PubMed  Google Scholar 

Xu W, Zhang X, Qian H, Zhu W, Sun X, Hu J, et al. Mesenchymal stern cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med. 2004;229(7):623–31.

Article  CAS  Google Scholar 

Kasas S, Wang X, Hirling H, Marsault R, Huni B, Yersin A, et al. Superficial and deep changes of cellular mechanical properties following cytoskeleton disassembly. Cell Motil Cytoskelet. 2005;62(2):124–32.

Article  CAS  Google Scholar 

Chen Y-J, Lin Y-H, Wu C-H, Hu J-L, Hwang L-C, Kang P-L, et al. Leveraging electric current stimulation and chemical induction to enhance MSC differentiation into cardiomyocytes. Regen Med. 2025. https://doi.org/10.1080/17460751.2025.2514905.

Article  PubMed  Google Scholar 

Fei W, Pang E, Hou L, Dai J, Liu M, Wang X, et al. Synergistic effect of hydrogen and 5-Aza on myogenic differentiation through the p38 MAPK signaling pathway in adipose-derived mesenchymal stem cells. Int J Stem Cells. 2023;16(1):78–92.

Article  CAS  PubMed  Google Scholar 

Gopalan C, Kirk E. Biology of cardiovascular and metabolic diseases. 1st ed. Cambridge (MA): Academic Press; 2022 Jan 11.

Babuin L, Jaffe AS. Troponin: the biomarker of choice for the detection of cardiac injury. CMAJ. 2005;173(10):1191–202.

Article  PubMed  PubMed Central  Google Scholar 

Kjenseth A, Fykerud TA, Sirnes S, Bruun J, Yohannes Z, Kolberg M, et al. The gap junction channel protein connexin 43 is covalently modified and regulated by SUMOylation. J Biol Chem. 2012;287(19):15851–61.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li W, Wu D, Niu Z, Jiang D, Ma H, He H, et al. 5-azacytidine suppresses EC9706 cell proliferation and metastasis by upregulating the expression of SOX17 and CDH1. Int J Mol Med. 2016;38(4):1047–54.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ghoshal K, Datta J, Majumder S, Bai S, Kutay H, Motiwala T, et al. 5-aza-deoxycytidine induces selective degradation of DNA methyltransferase 1 by a proteasomal pathway that requires the KEN box, bromo-adjacent homology domain, and nuclear localization signal. Mol Cell Biol. 2005. https://doi.org/10.1128/MCB.25.11.4727-4741.2005.

Article  PubMed  PubMed Central  Google Scholar 

Nakashima T, Ohkusa T, Okamoto Y, Yoshida M, Lee J-K, Mizukami Y, et al. Rapid electrical stimulation causes alterations in cardiac intercellular junction proteins of cardiomyocytes. American Journal of Physiology-Heart and Circulatory Physiology. 2014;306(9):H1324–33.

Article  CAS  PubMed  Google Scholar 

Cooper GM. Actin, myosin, and cell movement. In: The cell: A molecular approach. 2nd ed, Chapter 11. Sunderland (MA): Sinauer Associates; 2000.

Svitkina T. The actin cytoskeleton and actin-based motility. Cold Spring Harb Perspect Biol. 2018;10(1): a018267.

Article  PubMed  PubMed Central  Google Scholar