A human genome wide screen identifies miRNAs to increase recombinant Adeno-associated virus production in HEK293 cells

AAV

Adeno-associated virus

BAK1

Bcl-2 Homologous Antagonist/Killer

BAX

Apoptosis Regulator BAX

CFLAR

CASP8 and FADD-Like Apoptosis Regulator

DAVID

Database for Annotation, Visualization and Integrated Discovery

DDX3X

DEAD-Box Helicase 3 X-Linked

G3BP1

G3BP Stress Granule Assembly Factor 1

GFP

Green fluorescent protein

HCR-hAAT

Apolipoprotein E locus control region, human α1-antitrypsin

HEK293

Human embryonic kidney cells

HNRNPU

Heterogeneous Nuclear Ribonucleoprotein U

HpT

Hours after transfection

HuH-7 / Huh7

Human hepatocellular carcinoma cells

ITRs

Inverted terminal repeats

IQGAP1

IQ Motif Containing GTPase Activating Protein 1

JCRB

Japanese Collection of Research Bioresources

KEGG

Kyoto Encyclopedia of Genes and Genomes

MAVS

Mitochondrial Antiviral-Signaling Protein

Min, microRNA

minutesmiRNA / miR

NC

Negative cap-directed siRNA control

NT

Non-targeting siRNA control

PPIA

Peptidylprolyl isomerase A

QPCR

Quantitative polymerase chain reaction

RAAV

Recombinant Adeno-associated virus

Rep

AAV Replication-associated genes

RISC

RNA induced silencing complex

SEAP

Secreted embryonic alkaline phosphatase

SiRNA

Small interfering RNA

TBK1

Serine/Threonine-Protein Kinase TBK1

UT

Untransfected cells / untransduced cells

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