The nanofibers' interior of electrospun fibremats provides an effective platform for immobilizing sensor proteins for biomolecule detection. We developed fibremats with GINKO2-a fluorescent K⁺ sensor-encapsulated in nanofibres, enabling reversible K⁺ detection even with protease and real-time monitoring of K⁺ concentration changes secreted by chemically stimulated mammalian cells.The development of sensor devices that combine practical usability with high selectivity and sensitivity remains a pressing challenge in advancing artificial intelligence (AI)-based diagnostic technologies. These technologies rely on portable or implantable sensors to determine both the identity and concentration of biomolecules in biological fluids such as sweat, urine, or blood. However, biological fluids contain a wide variety of ions and molecules-from small metabolites to large macromolecules-beyond the specific analytes of interest. Thus, it is essential to selectively detect the target analyte amidst numerous interfering substances and to achieve accurate quantitative analysis. This requirement is particularly critical for implantable sensors and on-site analyses, where the laborious pretreatment steps commonly used in vitro assays, such as removal of interfering components, are impractical. In this context, protein-based sensors-engineered by fusing a signal emitting protein (e.g., a fluorescent protein) with a receptor protein that selectively binds the analytes-offer a rational solution. Such protein sensors have already been applied successfully for real-time, on-site detection of second messengers and metabolites including cAMP, lactate, and

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