Madea B (2016) Methods for determining time of death. Forensic Sci Med Pathol 12(4):451–485

Article  PubMed  Google Scholar 

Huang W, Zhao S, Liu H, Pan M, Dong H (2024) The role of protein degradation in Estimation postmortem interval and confirmation of cause of death in forensic pathology: A literature review. Int J Mol Sci 25(3):1659

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ehrenfellner B, Zissler A, Steinbacher P, Monticelli FC, Pittner S (2017) Are animal models predictive for human postmortem muscle protein degradation? Int J Legal Med 131(6):1615–1621

Article  PubMed  PubMed Central  Google Scholar 

Harvey ML, Gasz NE, Voss SC (2016) Entomology-based methods for estimation of postmortem interval. RRFMS 6:1–9

Article  Google Scholar 

Campobasso CP, Di Vella G, Introna F (2001) Factors affecting decomposition and Diptera colonization. Forensic Sci Int 120(1–2):18–27

Article  CAS  PubMed  Google Scholar 

Marrone A, La Russa D, Barberio L, Murfuni MS, Gaspari M, Pellegrino D (2023) Forensic proteomics for the discovery of new post mortem interval biomarkers: a preliminary study. IJMS. 27(19):14627

Piegari G, De Pasquale V, d’Aquino I, De Biase D, Caccia G, Campobasso CP et al (2023) Evaluation of muscle proteins for estimating the post-mortem interval in veterinary forensic pathology. Anim (Basel) 13(4):563

Google Scholar 

Choi KM, Zissler A, Kim E, Ehrenfellner B, Cho E, Lee S (2019) Postmortem proteomics to discover biomarkers for forensic PMI estimation. Int J Legal Med 133(3):899–908

Article  PubMed  PubMed Central  Google Scholar 

Pittner S, Gotsmy W, Zissler A, Ehrenfellner B, Baumgartner D, Schrüfer A et al (2020) Intra- and intermuscular variations of postmortem protein degradation for PMI estimation. Int J Legal Med 134(5):1775–82

Article  PubMed  PubMed Central  Google Scholar 

Pittner S, Ehrenfellner B, Monticelli FC, Zissler A, Sänger AM, Stoiber W et al (2016) Postmortem muscle protein degradation in humans as a tool for PMI delimitation. Int J Legal Med 130(6):1547–1555

Article  PubMed  PubMed Central  Google Scholar 

Zissler A, Ehrenfellner B, Foditsch EE, Monticelli FC, Pittner S (2018) Does altered protein metabolism interfere with postmortem degradation analysis for PMI estimation? Int J Legal Med 132(5):1349–56

Article  CAS  PubMed  PubMed Central  Google Scholar 

Geissenberger J, Pittner S, Ehrenfellner B, Jakob L, Stoiber W, Monticelli FC et al (2023) Effect of temporary freezing on postmortem protein degradation patterns. Int J Legal Med 137(6):1803–1814

Article  PubMed  PubMed Central  Google Scholar 

Pittner S, Merold V, Anders S, Lohner L, Amendt J, Klinger M et al (2022) A standard protocol for the analysis of postmortem muscle protein degradation: process optimization and considerations for the application in forensic PMI estimation. Int J Legal Med 136(6):1913–1923

Article  PubMed  PubMed Central  Google Scholar 

Battistini A, Capitanio D, Bailo P, Moriggi M, Tambuzzi S, Gelfi C et al (2023) Proteomic analysis by mass spectrometry of postmortem muscle protein degradation for PMI estimation: a pilot study. Forensic Sci Int 349:111774

Article  CAS  PubMed  Google Scholar 

Geissenberger J, Ehrenfellner B, Monticelli FC, Pittner S, Steinbacher P (2021) Dismembered porcine limbs as a proxy for postmortem muscle protein degradation. Int J Legal Med 135(4):1627–36

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mondello C, Stassi C, Minutoli L, Baldino G, Alibrandi A, Spatola GF et al (2021) Caspase 9 and caspase 3 immunohistochemical pattern in skeletal and cardiac muscles at different times after death: an experimental study on PMI Estimation. Diagnostics (Basel). 9(6):1062

Pittner S, Bugelli V, Weitgasser K, Zissler A, Sanit S, Lutz L et al (2020) A field study to evaluate PMI estimation methods for advanced decomposition stages. Int J Legal Med 134(4):1361–73

Article  PubMed  PubMed Central  Google Scholar 

Geissenberger J, Amendt J, Klampfer J, Thuemmel L, Jakob L, Monticelli FC et al (2024) Morphological changes and protein degradation during the decomposition process of pig cadavers placed outdoors or in tents-a pilot study. Forensic Sci Med Pathol 20(2):508–17

Article  CAS  PubMed  Google Scholar 

Pittner S, Bugelli V, Benbow ME, Ehrenfellner B, Zissler A, Campobasso CP et al (2020) The applicability of forensic time since death estimation methods for buried bodies in advanced decomposition stages. PLoS One 15(12):e0243395

Article  CAS  PubMed  PubMed Central  Google Scholar 

Brockbals L, Garrett-Rickman S, Fu S, Ueland M, McNevin D, Padula MP (2023) Estimating the time of human decomposition based on skeletal muscle biopsy samples utilizing an untargeted LC-MS/MS-based proteomics approach. Anal Bioanal Chem 415(22):5487–98

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pittner S, Ehrenfellner B, Zissler A, Racher V, Trutschnig W, Bathke AC et al (2017) First application of a protein-based approach for time since death estimation. Int J Legal Med 131(2):479–483

Article  PubMed  Google Scholar 

Sacco MA, Cordasco F, Scalise C, Ricci P, Aquila I (2022) Systematic review on post-mortem protein alterations: analysis of experimental models and evaluation of potential biomarkers of time of Death. Diagnostics (Basel). 17(6):1490

Secco L, Palumbi S, Padalino P, Grosso E, Perilli M, Casonato M et al (2025) Omics postmortem interval estimation: Syst Rev IJMS 26(3):1034

CAS  Google Scholar 

Zissler A, Stoiber W, Steinbacher P, Geissenberger J, Monticelli FC, Pittner S (2020) Postmortem protein degradation as a tool to estimate the PMI: a systematic review. Diagnostics (Basel) 10(12):1014

Article  CAS  PubMed  Google Scholar 

Jin Y, Peng Y, Lin Z, Chen YC, Wei L, Hacker TA et al (2016) Comprehensive analysis of tropomyosin isoforms in skeletal muscles by top-down proteomics. J Muscle Res Cell Motil 37(1–2):41–52

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dawidowska J, Krzyżanowska M, Markuszewski MJ, Kaliszan M (2021) The application of metabolomics in forensic science with focus on forensic toxicology and time-of-death estimation. Metabolites 11(12):801

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rhein M, Hagemeier L, Klintschar M, Muschler M, Bleich S, Frieling H (2015) DNA methylation results depend on DNA integrity – role of post mortem interval. Front Genet [Internet]. [cited 2025 Dec 11];6. Available from: https://www.frontiersin.org/journals/genetics/articles/https://doi.org/10.3389/fgene.2015.00182/full

Sampaio-Silva F, Magalhães T, Carvalho F, Dinis-Oliveira RJ, Silvestre R (2013) Profiling of RNA degradation for estimation of post morterm interval. PLoS One 8(2):e56507

Article  CAS  PubMed  PubMed Central  Google Scholar 

Peng D, Lv M, Li Z, Tian H, Qu S, Jin B et al (2020) Postmortem interval determination using mRNA markers and DNA normalization. Int J Legal Med 134(1):149–157

Article  PubMed  Google Scholar 

Tu C, Du T, Ye X, Shao C, Xie J, Shen Y (2019) Using MiRNAs and circrnas to estimate PMI in advanced stage. Leg Med (Tokyo) 38:51–57

Article  CAS  PubMed  Google Scholar 

Bauer M, Gramlich I, Polzin S, Patzelt D (2003) Quantification of mRNA degradation as possible indicator of postmortem interval—a pilot study. Leg Med 5(4):220–227

Article  CAS  Google Scholar 

Heinrich M, Matt K, Lutz-Bonengel S, Schmidt U (2007) Successful RNA extraction from various human postmortem tissues. Int J Legal Med 121(2):136–142

Article  PubMed  Google Scholar 

Tozzo P, Scrivano S, Sanavio M, Caenazzo L (2020) The role of DNA degradation in the estimation of post-mortem interval: a systematic review of the current literature. Int J Mol Sci 21(10):3540

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sacco MA, Aquila I (2023) Proteomics a new research frontier in forensic pathology. Int J Mol Sci 24(13):10735

Frankenfield AM, Ni J, Ahmed M, Hao L (2022) Protein contaminants matter: Building universal protein contaminant libraries for DDA and DIA proteomics. J Proteome Res 21(9):2104–2113

Hodge K, Have ST, Hutton L, Lamond AI (2013) Cleaning up the masses: exclusion lists to reduce contamination with HPLC-MS/MS. J Proteome 88:92–103