Prokaryotes are one of the diverse and complex life forms, and prokaryotic taxonomy is essential for the determination of prokaryotic classification and effective communication within the scientific community (Jiao et al., 2024). The 16S rRNA gene sequence, due to its high sequence conservation, was proposed by Woese to provide insights into the determination of evolutionary and phylogenetic relationships of prokaryotes (Woese and Fox, 1977; Lane et al., 1985; Olsen and Woese, 1993; Woese, 1987), and it still is a fundamental reference for phylogenetic analysis.

The List of Prokaryotic Names with Standing in Nomenclature (LPSN) (Parte, 2018; Parte, 2014; Parte et al., 2020), is a comprehensive, robust, and up-to-date database that presents name lists, validation status, etymology details, and URL links of 16S rRNA gene sequences of the type strains according to the International Code of Nomenclature of Prokaryotes (ICNP) (Int. J. Syst. Evol. Microbiol., 2019), making it an essential and solid reference resource and supportive databank for prokaryotic taxonomists.

Nevertheless, the collection of taxonomic information of type strains and their 16S rRNA gene sequences from the LPSN database, and the summarization of the validation status of specific taxa referring to the LPSN, is often manual, laborious, and time-consuming.

While the LPSN-API (https://lpsn.dsmz.de/text/lpsn-api) facilitates the collection of sequence information and related target data from the LPSN database, it is still not convenient for users with no experience in R or Python programming, as the LPSN-API tool requires proficiency and experience in R or Python programming. In addition, downloaded sequences require further management, including combination and formatting for the construction of the 16S rRNA gene sequence-based phylogenetic trees. Especially, while constructing phylogenetic trees for a dozen to hundreds of newly discovered potential species (Liu et al., 2020; Liu et al., 2021; Forster et al., 2019), it is often essential to download 16S rRNA gene sequences of all members within a genus to assess the phylogenetic and evolutionary relationships. For any genus containing a large number of species, such as Clostridium, which includes 163 child taxa with validly published and correct names, downloading the 16S rRNA gene sequences of all these members one by one, then standard formatting them, is a time-consuming and labor-intensive process. Although it is a simple and repetitive task, it still requires significant effort and time for most microbiologists who are not fluent with batch processing tools like Bash.To streamline and batch automation of this process, we developed LPSN-API Assistant 1.0 (https://gitee.com/rashidin-abdugheni/lpsn-api_-assistant_1.0), a standalone executable tool with a user interface (UI), which provides convenient access to the LPSN API and includes the taxonomic information collection, sequence merging, and formatting functions. For the current version, we generated a standalone executable for Windows since Windows is the most prevalent operating system. This tool is designed to automate the taxonomic information collection, bulk download, and organization of 16S rRNA gene sequences of valid type strains of any target genus from LPSN, along with their associated data, including details of the child taxa and validation status.