Vascularization and maturation of kidney organoids: a comprehensive review of technological trends and challenges

GBD Chronic Kidney Disease Collaboration. Global, regional, and national burden of chronic kidney disease, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet. 2020;395(10225):709–33.

Article  Google Scholar 

Vanslambrouck JM, Tan KS, Mah S, Little MH. Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells. Nat Protoc. 2023;18(11):3229–52.

Article  CAS  PubMed  Google Scholar 

Sharmin S, Taguchi A, Kaku Y, Yoshimura Y, Ohmori T, et al. Human induced pluripotent stem cell-derived podocytes mature into vascularized glomeruli upon experimental transplantation. J Am Soc Nephrol. 2016;27(6):1778–91.

Article  CAS  PubMed  Google Scholar 

Homan KA, Gupta N, Kroll KT, Kolesky DB, Skylar-Scott M, et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro. Nat Methods. 2019;16(3):255–62.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Konoe R, Morizane R. Strategies for improving vascularization in kidney organoids: a review of current trends. Biology. 2023;12(4):503.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Schumacher A, Roumans N, Rademakers T, Joris V, Eischen-Loges MJ, van Griensven M, et al. Enhanced microvasculature formation and patterning in iPSC-derived kidney organoids cultured in physiological hypoxia. Front Bioeng Biotechnol. 2022;10:860138.

Article  PubMed  PubMed Central  Google Scholar 

Maggiore JC, LeGraw R, Przepiorski A, Velazquez J, Chaney C, et al. A genetically inducible endothelial niche enables vascularization of human kidney organoids with multilineage maturation and emergence of renin expressing cells. Kidney Int. 2024;106(6):1086–100.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Koning M, Dumas SJ, Avramut MC, Koning RI, Meta E, et al. Vasculogenesis in kidney organoids upon transplantation. NPJ Regen Med. 2022;7(1):40.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kroll KT, Homan KA, Uzel SGM, Mata MM, Wolf KJ, et al. A perfusable, vascularized kidney organoid-on-chip model. Biofabrication. 2024;16(4):045003.

Article  CAS  Google Scholar 

Nishimura Y. Revolutionizing renal research: the future of kidney-on-a-chip in biotechnology. Regen Ther. 2024;26:275–80.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wörsdörfer P, Ergün S. The impact of oxygen availability and multilineage communication on organoid maturation. Antioxid Redox Signal. 2021;35(3):217–33.

Article  PubMed  Google Scholar 

Wang R, Sui Y, Liu Q, Xiong Y, Li S, et al. Recent advances in extracellular matrix manipulation for kidney organoid research. Front Pharmacol. 2024;15:1472361.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jansen J, van den Berge BT, van den Broek M, Maas RJ, Daviran D, et al. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling. Development. 2022;149(9):dev200198.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Khoshdel Rad N, Aghdami N, Moghadasali R. Cellular and molecular mechanisms of kidney development: from the embryo to the kidney organoid. Front Cell Dev Biol. 2020;8:183.

Article  PubMed  PubMed Central  Google Scholar 

Sahara Y, Fukui C, Kuniyoshi Y, Takasato M. Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids. Commun Biol. 2024;7(1):1532.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gao Z, Chen X. Fatty acid β-oxidation in kidney diseases: perspectives on pathophysiological mechanisms and therapeutic opportunities. Front Pharmacol. 2022;13:805281.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nishimura Y, Wang PC. Possibility of culturing the early developing kidney cells by utilizing simulated microgravity environment. Biochem Biophys Res Commun. 2021;573:9–12.

Article  CAS  PubMed  Google Scholar 

Nishimura Y. Technology using simulated microgravity. Regen Ther. 2023;24:318–23.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Aceves JO, Heja S, Kobayashi K, Robinson SS, Miyoshi T, et al. 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake. Sci Rep. 2022;12(1):14997.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee HN, Choi YY, Kim JW, Lee YS, Choi JW, et al. Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip. Nano Converg. 2021;8(1):35.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gao Q, Wang J, Zhang H, Wang J, Jing Y, et al. Organoid vascularization: strategies and applications. Adv Healthc Mater. 2025. https://doi.org/10.1002/adhm.202500301.

Article  PubMed  PubMed Central  Google Scholar 

Raykhel I, Nishikawa M, Sakai Y, Vainio SJ, Skovorodkin I. Vascularization of kidney organoids: different strategies and perspectives. Front Urol. 2024;4:1355042.

Article  PubMed  PubMed Central  Google Scholar 

Shaw I, Rider S, Mullins J, Hughes J, Péault B. Pericytes in the renal vasculature: roles in health and disease. Nat Rev Nephrol. 2018;14(8):521–34.

Article  CAS  PubMed  Google Scholar 

Bas-Cristóbal Menéndez A, Du Z, van den Bosch TPP, Othman A, Gaio N, et al. Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Sci Rep. 2022;12(1):20699.

Article  PubMed  PubMed Central  Google Scholar 

van den Berg CW, Dumas SJ, Little MH, Rabelink TJ. Challenges in maturation and integration of kidney organoids for stem cell-based renal replacement therapy. Kidney Int. 2025;107(2):262–70.

Article  PubMed  Google Scholar 

Geuens T, van Blitterswijk CA, LaPointe VLS. Overcoming kidney organoid challenges for regenerative medicine. NPJ Regen Med. 2020;5:8.

Article  PubMed  PubMed Central  Google Scholar 

McDonough AA, Foley TS, Ralph DL, Schwindt S, Soong J, et al. Effect of sex chromosome complement versus gonadal hormones on abundance of renal transporters. Am J Physiol Renal Physiol. 2025;328(5):F638–46.

CAS  PubMed  PubMed Central  Google Scholar 

Lichner Z, Mac-Way F, Yousef GM. Obstacles in renal regenerative medicine: metabolic and epigenetic parallels between cellular reprogramming and kidney cancer oncogenesis. Eur Urol Focus. 2019;5(2):250–61.

Article  PubMed  Google Scholar 

Romero-Guevara R, Ioannides A, Xinaris C. Kidney organoids as disease models: strengths, weaknesses and perspectives. Front Physiol. 2020;11:563981.

Article  PubMed  PubMed Central  Google Scholar 

Przepiorski A, Sander V, Tran T, Hollywood JA, Sorrenson B, et al. A simple bioreactor-based method to generate kidney organoids from pluripotent stem cells. Stem Cell Rep. 2018;11(2):470–84.

Article  CAS  Google Scholar 

Nauryzgaliyeva Z, Goux Corredera I, Garreta E, Montserrat N. Harnessing mec

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