Interplay between miR-21 and mTOR signaling in an experimental mouse model of polycystic ovary syndrome

Abbott DHTA, Dumesic DA (2009) Fetal, infant, adolescent and adult phenotypes of polycystic ovary syndrome in prenatally androgenized female rhesus monkeys. Am J Primatol 71:776–784

Article  CAS  PubMed  PubMed Central  Google Scholar 

Adhikari D, Liu K (2009) Molecular mechanisms underlying activation of mammalian primordial follicles. Endocr Rev 30(5):438–464

Article  CAS  PubMed  Google Scholar 

Ahmed AI, Dowidar MF, Negm AF, Abdellatif H, Alanazi A, Alassiri M et al (2024) Bone marrow mesenchymal stem cells expressing Neat-1, Hotair-1, miR-21, miR-644, and miR-144 subsided cyclophosphamide-induced ovarian insufficiency by remodeling the IGF-1-kisspeptin system, ovarian apoptosis, and angiogenesis. J Ovarian Res. ;17(1):184. https://doi.org/10.1186/s13048-024-01498-x. Erratum in: J Ovarian Res. 2024;17(1):239. doi: 10.1186/s13048-024-01564-4. PMID: 39267091; PMCID: PMC11396253

Almahbobi G, Anderiesz C, Hutchinson P, McFarlane JR, Wood C, Trounson AO (1996) Functional integrity of granulosa cells from polycystic ovaries. Clin Endocrinol 44:5

Article  Google Scholar 

Anderson E, Lee GY (1997) The polycystic ovarian condition: apoptosis and epithelialization of follicles in the DHEA-treated rat model. Tissue Cell 29(2):171–189

Article  CAS  PubMed  Google Scholar 

Baley J, Li J (2012) MicroRNAs and ovarian function. J Ovarian Res 5:8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bartel DP, Otsuka M, Lin F, McAllister JM (2004) Foundational studies on microRNA biogenesis and ovarian function. Cell 116(2):281–297

Article  CAS  PubMed  Google Scholar 

Bartolucci A, Uliasz TF, Peluso JJ (2020) MicroRNA-21 as a regulator of human cumulus cell viability and its potential influence on the developmental potential of the oocyte. Biol Reprod 103(1):94–103. https://doi.org/10.1093/BIOLRE/IOAA058

Article  PubMed  Google Scholar 

Blank SK, McCartney CR, Marshall JC (2006) The origins and sequelae of abnormal neuroendocrine function in PCOS. Hum Reprod Update 12(4):351–361

Article  CAS  PubMed  Google Scholar 

Bosch E, Alviggi C, Lispi M, Conforti A, Hanyaloglu AC, Chuderland D et al (2021) Reduced FSH and LH action: implications for medically assisted reproduction. Hum Reprod 36(6):1469–1480

Article  CAS  PubMed  PubMed Central  Google Scholar 

Byers SL, Wiles MV, Dunn SL, Taft RA (2012) Mouse estrous cycle identification using vaginal cytology. PLoS ONE 7(4):e35538

Article  CAS  PubMed  PubMed Central  Google Scholar 

Carletti MZ, Christenson LK (2010) MicroRNA-21 prevents apoptosis in granulosa cells. Endocrinology 151(3):1331–1341

Google Scholar 

Carletti MZ, Fiedler SD, Christenson LK (2010) MicroRNA 21 blocks apoptosis in mouse periovulatory granulosa cells. Biol Reprod 83(2):286–295. https://doi.org/10.1095/biolreprod.109.081448Epub 2010 Mar 31. PMID: 20357270; PMCID: PMC2907287

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cheng Y, Kim J, Li XX, Hsueh AJW (2015) Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators. PLoS ONE 10(2). https://doi.org/10.1371/JOURNAL.PONE.0117769

Coffler MS, Patel KS, Dahan MH, Malcom PJ, Kawashima T, Deutsch R, Chang RJ (2003) Evidence for abnormal granulosa cell responsiveness to follicle stimulating hormone in women with polycystic ovary syndrome. J Clin Endocrinol Metab 88:1742–1747

Article  CAS  PubMed  Google Scholar 

Ding S, Wang P (2025) The Life of MicroRNAs: biogenesis, function and decay in cancer. Biomolecules 15(10):1393. https://doi.org/10.3390/biom15101393PMID: 41154621; PMCID: PMC12562231

Article  CAS  PubMed  PubMed Central  Google Scholar 

Dou P, Zhang TT, Xu Y, Xue Q, Zhang Y, Shang J, Yang XL (2024) A randomized trial of the efficacy of three weight loss diet interventions in overweight/obese with polycystic ovary syndrome. Endocr Metab Immune Disord Drug Targets 24(14):1686–1697

Article  CAS  PubMed  Google Scholar 

Franks S, Gilling-Smith C, Watson H, Willis D (1999) Insulin action in the normal and polycystic ovary. Endocrinol Metab Clin North Am 28(2):361–378

Article  CAS  PubMed  Google Scholar 

Franks S, McCarthy MI, Hardy K (2006) Development of polycystic ovary syndrome: involvement of genetic and environmental factors. Int J Androl 29(1):278–285

Article  CAS  PubMed  Google Scholar 

Franks S, Webber LJ, Stark J, Hardy K (2009) Theca-cell androgen excess and steroidogenic dysregulation in PCOS. Endocr Rev 30(2):193–208

Google Scholar 

Garcia-Martinez JM, Alessi DR (2008) mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1). Biochem J 416(3):375–385

Article  CAS  PubMed  Google Scholar 

Guo R, Zheng H, Li Q, Qiu X, Zhang J, Cheng Z (2022) Melatonin alleviates insulin resistance through the PI3K/AKT signaling pathway in ovary granulosa cells of polycystic ovary syndrome. Reprod Biol 22(1):100594. https://doi.org/10.1016/j.repbio.2021.100594Epub 2021 Dec 22. PMID: 34953312

Article  CAS  PubMed  Google Scholar 

He Y, Peng X, Wu T, Yang W, Liu W, Zhang J, Su Y, Kong F, Dou X, Li J (2017) Restricting the induction of NGF in ovarian stroma engenders selective follicular activation through the mTOR signaling pathway. Cell Death Dis 8:2817

Article  Google Scholar 

Huffman AM, Rezq S, Basnet J, Yanes Cardozo LL, Romero DG (2022) MicroRNA-21 Genetic Ablation Exacerbates Insulin Signaling Dysregulation in Hyperandrogenemic Female Mice. FASEB J 36. https://doi.org/10.1096/fasebj.2022.36.s1.r4063

Imbar T, Eisenberg I (2014) Regulatory role of microRNAs in ovarian function. Fertil Steril 101:1524–1530

Article  CAS  PubMed  Google Scholar 

Jarrett BY, Vanden Brink H, Oldfield AL, Lujan ME (2020) Ultrasound characterization of disordered antral follicle development in women with polycystic ovary syndrome. J Clin Endocrinol Metab 105(11):e3847–e3861. https://doi.org/10.1210/clinem/dgaa515PMID: 32785651; PMCID: PMC7473602

Article  PubMed  PubMed Central  Google Scholar 

Jiang L-Y, Li W, Wu M-M, Cao S-F (2015) Ciculating miRNA-21 as a Biomarker predicts polycystic ovary syndrome (PCOS) in patients. Clin Lab 61(8):1009–1015. https://doi.org/10.7754/CLIN.LAB.2015.150122)

Article  CAS  PubMed  Google Scholar 

Joham AE, Tay CT, Laven J, Louwers YV, Azziz R (2025) Approach to the patient: diagnostic challenges in the workup for polycystic ovary syndrome. J Clin Endocrinol Metab 110(7):e2298–e2308. https://doi.org/10.1210/clinem/dgae910PMID: 39836632; PMCID: PMC12187463

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kafali H, Iriadam M, Ozardali I, Demir N (2004) Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease. Arch Med Res 35(2):103–108

Article  CAS  PubMed  Google Scholar 

Kerr JB, Myers M, Anderson RA (2013) The dynamics of the primordial follicle reserve. Reproduction 146:205–215

Article  Google Scholar 

Khodarahmi P (2023) Evaluation of the potential of miR-21 as a diagnostic marker for oocyte maturity and embryo quality in women undergoing ICSI. Dent Sci Rep 13(1). https://doi.org/10.1038/s41598-023-28686-x

Laplante M, Sabatini DM (2012) mTOR signaling in growth control and disease. Cell 149(2):274–293

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lentini G, Querqui A, Monti N, Bizzarri M (2025) PCOS and inositols–advances and lessons we are learning. A narrative review. Drug Des Devel Ther 19:4183–4199

Article  PubMed  PubMed Central  Google Scholar 

Li D, Li C, Xu Y, Xu D, Li H, Gao L et al (2016) Differential expression of microRNAs in the ovaries from letrozole-induced rat model of polycystic ovary syndrome. DNA Cell Biol 35(4):177–183 Epub 2016 Jan 8. PMID: 26745201

Article  CAS  PubMed  Google Scholar 

Liu Z, Rudd MD, Hernandez-Gonz

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