Willey JC, Crawford EL, Craig DJ, Morrison TM, He HJ, Herman JG. Abstract 7024: development of quality control methods for reliable NGS measurement of methylation in cfDNA. Cancer Res. 2024;84:7024. https://doi.org/10.1158/1538-7445.Am2024-7024.
Gunter HM, Youlten SE, Reis ALM, McCubbin T, Madala BS, Wong T, et al. A universal molecular control for DNA, mRNA and protein expression. Nat Commun. 2024;15:2480. https://doi.org/10.1038/s41467-024-46456-9.
Article CAS PubMed PubMed Central Google Scholar
Rascón-Durán ML, Corella-Madueño MAG, Pérez-Martínez CJ, Anduro-Corona I, García-Alegría AM, Astiazaran-Garcia H. Quantification of DNA through the NanoDrop spectrophotometer: methodological validation using standard reference material and Sprague Dawley rat and human DNA. Int J Anal Chem. 2020. https://doi.org/10.1155/2020/8896738.
Article PubMed PubMed Central Google Scholar
Deveson IW, Chen WY, Wong T, Hardwick SA, Andersen SB, Nielsen LK, et al. Representing genetic variation with synthetic DNA standards. Nat Methods. 2016;13:784–91. https://doi.org/10.1038/Nmeth.3957.
Article CAS PubMed Google Scholar
Reis ALM, Deveson IW, Wong T, Madala BS, Barker C, Blackburn J, et al. A universal and independent synthetic DNA ladder for the quantitative measurement of genomic features. Nat Commun. 2020;11:3609. https://doi.org/10.1038/s41467-020-17445-5.
Article CAS PubMed PubMed Central Google Scholar
Traylor A, Lee PW, Hsieh K, Wang TH. Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration. Anal Chim Acta. 2024;1297:342371. https://doi.org/10.1016/j.aca.2024.342371.
Article CAS PubMed PubMed Central Google Scholar
Lee DH, Yoo HB, Hong KS, Park SR, Jeong S, Yang I. Development of gene-in-plasmid DNA reference materials certified by single-molecule counting. Anal Bioanal Chem. 2025;417:2489–501. https://doi.org/10.1007/s00216-024-05675-1.
Article CAS PubMed Google Scholar
Qu W, Wang F, Sun D, Liu Y, Jin X, Gong Z, et al. Internal validation of the GA118-24B genetic analyzer, a stable capillary electrophoresis system for forensic DNA identification. Int J Legal Med. 2024;138:361–73. https://doi.org/10.1007/s00414-023-03106-x.
Yoo HB, Park SR, Dong L, Wang J, Sui Z, Pavšič J, et al. International comparison of enumeration-based quantification of DNA copy-concentration using flow cytometric counting and digital polymerase chain reaction. Anal Chem. 2016;88:12169–76. https://doi.org/10.1021/acs.analchem.6b03076.
Article CAS PubMed Google Scholar
Singer VL, Jones LJ, Yue ST, Haugland RP. Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantitation. Anal Biochem. 1997;249:228–38.
Article CAS PubMed Google Scholar
Cavaluzzi MJ, Borer PN. Revised UV extinction coefficients for nucleoside-5’-monophosphates and unpaired DNA and RNA. Nucleic Acids Res. 2004;32:e13. https://doi.org/10.1093/nar/gnh015.
Article PubMed PubMed Central Google Scholar
Somanath Bhat NS, Mostyn T, Bains GS, Griffiths KR, Emslie KR. Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units. Anal Chem. 2010;82:7185–92.
Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makarewicz AJ, et al. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number. Anal Chem. 2011;83:8604–10.
Article CAS PubMed PubMed Central Google Scholar
Whale AS, Huggett JF, Cowen S, Speirs V, Shaw J, Ellison S, et al. Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation. Nucleic Acids Res. 2012;40:e82. https://doi.org/10.1093/nar/gks203.
Article CAS PubMed PubMed Central Google Scholar
Dong L, Wang X, Wang S, Du J, Niu C, Yang J, et al. Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR. Talanta. 2021;224:121726. https://doi.org/10.1016/j.talanta.2020.121726.
Article CAS PubMed Google Scholar
Dong L, Wang J, Li L, Wang S, Yang J, Niu C, et al. CCQM-K176 breast cancer biomarker HER2 copy number variation (CNV) measurement. Metrologia. 2024;61:08017. https://doi.org/10.1088/0026-1394/61/1a/08017.
Dong L, Meng Y, Sui Z, Wang J, Wu L, Fu B. Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material. Sci Rep. 2015;5:13174. https://doi.org/10.1038/srep13174.
Article CAS PubMed PubMed Central Google Scholar
Cottenet G, Blancpain C, Chuah PF. Performance assessment of digital PCR for the quantification of GM-maize and GM-soya events. Anal Bioanal Chem. 2019;411:2461–9. https://doi.org/10.1007/s00216-019-01692-7.
Article CAS PubMed Google Scholar
Trypsteen W. The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020. Clin Chem. 2020;66:1012.
Whale AS, Cowen S, Foy CA, Huggett JF. Assessment of digital PCR as a primary reference measurement procedure to support advances in precision medicine. Clin Chem. 2018;64:1296–307. https://doi.org/10.1373/clinchem.2017.285478.
Article CAS PubMed Google Scholar
Wang X, Bae YK, Hong SR, Yang I, Shibayama S, Kato M, et al. Development and interlaboratory validation of a linearized plasmid DNA certified reference material by single molecule direct counting and digital PCR. Talanta. 2026. https://doi.org/10.1016/j.talanta.2025.128762.
Article PubMed PubMed Central Google Scholar
Yen GS, Fujimoto BS, Schneider T, Kreutz JE, Chiu DT. Statistical analysis of non-uniform volume distributions for droplet-based digital PCR assays. J Am Chem Soc. 2019;141:1515–25.
Article CAS PubMed PubMed Central Google Scholar
Niu C, Dong L, Wang S, Yang J, Gao Y. Development of certified reference material of Japanese encephalitis virus by reverse transcription digital PCR and high-performance liquid chromatography-isotope dilution mass spectrometry. Microchem J. 2025;219:116204. https://doi.org/10.1016/j.microc.2025.116204.
Niu C, Wang X, Gao Y, Qiao X, Xie J, Zhang Y, et al. Accurate quantification of SARS-CoV-2 RNA by isotope dilution mass spectrometry and providing a correction of reverse transcription efficiency in droplet digital PCR. Anal Bioanal Chem. 2022;414:6771–7. https://doi.org/10.1007/s00216-022-04238-6.
Article CAS PubMed PubMed Central Google Scholar
Shibayama S, Fujii S, Inagaki K, Yamazaki T, Yoshioka M, Takatsu A. Development of certified reference material NMIJ CRM 6205-a for the validation of DNA quantification methods: accurate mass concentrations of 600-bp DNA solutions having artificial sequences. Anal Bioanal Chem. 2019;411:6091–100. https://doi.org/10.1007/s00216-019-01992-y.
Article CAS PubMed Google Scholar
Lowenthal MS, Quittman E, Phinney KW. Absolute quantification of RNA or DNA using acid hydrolysis and mass spectrometry. Anal Chem. 2019;91:14569–76. https://doi.org/10.1021/acs.analchem.9b03625.
Article CAS PubMed PubMed Central Google Scholar
Tu Q, Guidry EN, Meng FY, Wang TB, Gong XY. A high-throughput flow injection inductively coupled plasma mass spectrometry method for quantification of oligonucleotides. Microchem J. 2016;124:668–74. https://doi.org/10.1016/j.microc.2015.10.011.
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