Sayyad SF, Randive DS, Jagtap SM, Chaudhari SR, Panda BP (2012) Preparation and evaluation of fermented Ayurvedic formulation: Arjunarishta. J Appl Pharm Sci 2(5):122–124. https://doi.org/10.7324/JAPS.2012.2521

Article  Google Scholar 

Sharma S, Modi K, Shah M (2024) Development and validation of high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography (HPLC) methods for the simultaneous determination of myricetin and quercetin in Manilkara hexandra. JPC-J Planar Chromat 37:511–519. https://doi.org/10.1007/s00764-024-00325-9

Article  CAS  Google Scholar 

Meena AK, Rao MM, Panda P, Kiran YA, Singh Uttam SB (2010) Standardization of ayurvedic polyherbal formulation, pancasama churna. Int J Pharmacogn Phytochem Res 2(1):11–14

Google Scholar 

Singh B, Singh H, Kaur S, Singh B (2026) Preclinical pharmacology studies of zingerone with special reference to potential therapeutic applications. World J Immunol 16(1):111511

Article  Google Scholar 

Hasan FA, Ibrahim AU, Suer K, Asir S (2026) The effect of variations in temperature and contact time of zingerone, [6]-gingerol and shogaol as disinfectants on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Microorganisms 14(3):539

Article  PubMed  PubMed Central  Google Scholar 

Nurmansyah A, Shiyan S, Arina Y, Sopyan I, Muchtaridi M (2026) Chemometric classification of red ginger (Zingiber officinale var. rubrum) oleoresin from different regions using Fourier-transform infrared-ATR spectroscopy. J Adv Pharm Technol Res 17(1):51–58

Article  PubMed  PubMed Central  Google Scholar 

Waghmare P, Diwane C, Jadhav P, Patil V, Barhe P (2022) An overview on ‘Zingiber officinale’ (ginger). World J Pharm Res 11(4):625–646. https://doi.org/10.20959/wjpr20224-23568

Article  CAS  Google Scholar 

Mendes AG, Campos CD, Pereira-Filho JL, Almeida VS, Moreira IV, Marques RF, Silva MC, Monteiro-Neto V (2026) Ellagic acid as a promising antifungal agent: a review of mechanisms, synergy, and formulation strategies. Antibiotics (Basel) 15(1):72

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tang Y, Wang C, Li Z, Wang L, Guo H, Gao Q (2026) Preclinical evidence and potential mechanisms of ellagic acid in animal models of Parkinson’s disease: a systematic review and meta-analysis. ACS Nutr Sci 1(2):1–17. https://doi.org/10.1021/acsnutrsci.5c00024

Article  CAS  Google Scholar 

Rajput AH, Gavali RD, Jadhav AP (2024) Development and validation of a novel high-performance thin-layer chromatography method for the quantitative estimation of zingerone. JPC-J Planar Chromat 37(1):87–93. https://doi.org/10.1007/s00764-023-00268-7

Article  CAS  Google Scholar 

Bseiso Y, Alotaibi BS, Aljabali AA, Gammoh O, Rabeh ME, Rabi AM, Qnais E, Alqudah A (2026) Evaluation of scopoletin as a potential therapeutic agent in fibromyalgia: evidence from a reserpine-induced mouse model. Behav Pharmacol 37(2):85–96

Article  CAS  PubMed  Google Scholar 

Sani A, Liu C, Azam J, Khan S (2026) Xanthoangelol mitigates Neuroinflammation and Hypoxia via MAPK and HIF-1 signaling modulation: network pharmacology and in-vivo approach in a haloperidol-induced model of Parkinson’s disease. Res Sq. https://doi.org/10.21203/rs.3.rs-8572610/v1

Article  PubMed  PubMed Central  Google Scholar 

Guo F, Zhou H, Yan F, Chen J, Zhu Q, Tan Y, Guo Y, Lai X, Li Z, Zhang M, Ni Y (2026) A comprehensive multiomics approach illuminates the biosynthetic mechanism of scoparone in Artemisia capillaris. Plant Biotechnol J 24(1):346–360

Article  CAS  PubMed  Google Scholar 

Bulbul MR, Chowdhury MN, Naima TA, Sami SA, Imtiaj MS, Huda N, Uddin MG (2022) A comprehensive review on the diverse pharmacological perspectives of Terminalia chebula Retz. Heliyon 8(8):88–95. https://doi.org/10.1016/j.heliyon.2022.e10220

Article  CAS  Google Scholar 

Pallavi R, Jha S (2021) Validated quantification of gallic acid and ellagic acid in Triphala using high-performance thin-layer chromatography. JPC–J Planar Chromat 34(5):447–453. https://doi.org/10.1007/s00764-021-00130-8

Article  CAS  Google Scholar 

Ghasemzadeh A, Jaafar HZE, Rahmat A (2010) Identification and concentration of bioactive compounds in Zingiber officinale using HPLC analysis. Molecules 15(9):6231–6243. https://doi.org/10.3390/molecules15096231

Article  CAS  PubMed  PubMed Central  Google Scholar 

Thorat N, Dodiya T, Prajapati D (2022) HPTLC method development and validation for simultaneous estimation of berberine, ellagic acid and ferulic acid in Amrtadi Churna. J Nat Remedies 22(4):649–657. https://doi.org/10.18311/jnr/2022/31350

Article  Google Scholar 

Shaha M, Patel HU, Akabari AH (2024) Eco-friendly HPTLC method for simultaneous estimation of gallic acid, ellagic acid and curcumin in herbal formulation. Essent Chem 1(1):1–10. https://doi.org/10.1080/28378083.2024.2420104

Article  Google Scholar 

Meier B, Spriano D (2010) Modern HPTLC—a perfect tool for quality control of herbals and their preparations. J AOAC Int 93(5):1399–1409. https://doi.org/10.1093/jaoac/93.5.1399

Article  CAS  PubMed  Google Scholar 

Ghozzy EA, El-Enany NM, Tolba MM, El Abass SA (2024) An eco-friendly and cost-effective HPTLC method for quantification of COVID-19 antiviral drug and co-administered medications in spiked human plasma. Sci Rep 14(1):10025. https://doi.org/10.1038/s41598-024-56923-4

Article  CAS  PubMed  PubMed Central  Google Scholar 

Benefits of HPTLC. https://camag.com/industries-and-application/benefits-of-hptlc/. Accessed 24 May 2026

Sanganalmath PU, Sujatha KM, Bhargavi SM et al (2009) Simple, accurate and rapid HPTLC method for analysis of theophylline in post-mortem blood and validation of the method. J Planar Chromatogr Mod TLC 22:29–33. https://doi.org/10.1556/JPC.22.2009.1.6

Article  CAS  Google Scholar 

Zhang SM, Wang XF, Feng J, Sun ZL (2016) Chemical constituents of the seeds of Celosia cristata. Chem Nat Compd 52(5):827–829. https://doi.org/10.1007/s10600-016-1788-z

Article  CAS  Google Scholar 

Kurangi B, Jalalpure S (2020) A validated stability-indicating RP-HPLC method for piperine estimation in black pepper, marketed formulation and nanoparticles. Indian J Pharm Educ Res 54(3):677–686. https://doi.org/10.5530/ijper.54.3s.168

Article  CAS  Google Scholar 

Adhikari T, Saha P (2022) Quantitative estimation of immunomodulatory flavonoid quercetin by HPTLC in different leafy vegetables available in West Bengal. Pharmacogn Res 14(4):543–549. https://doi.org/10.5530/pres.14.4.62

Article  CAS  Google Scholar 

Lalwani R, Bodiwala K, Gajjar A (2025) High-performance thin-layer chromatography method for the quantitative assessment of caffeic acid, coumaric acid, and ferulic acid in ethanol extracts of Kigelia africana fruits. J Planar Chromatogr Mod TLC 38:147–154. https://doi.org/10.1007/s00764-025-00368-6

Article  CAS  Google Scholar 

Khan W, Chester K, Anjum V, Ahmad W, Ahmad S, Narwaria A, Kumar DP, Katiyar CK (2017) Chromatographic profiling of Pancharishta at different stages of its development using HPTLC, HPLC, GC–MS and UPLC–MS. Phytochem Lett 20:391–400. https://doi.org/10.1016/j.phytol.2017.04.034

Article  CAS  Google Scholar 

Pozharitskaya ON, Ivanova SA, Shikov AN, Makarov VG (2006) Separation and quantification of terpenoids of Boswellia serrata Roxb. extract by planar chromatography techniques (TLC and AMD). J Sep Sci 29(14):2245–2250. https://doi.org/10.1002/jssc.200600078

Article  CAS  PubMed  Google Scholar 

ICMR (2012) Gingerol. Phytochemical reference standards of selected Indian medicinal plants, vol 2. Indian Council of Medical Research, New Delhi, pp 163–164

Google Scholar 

Chen CC, Rosen RT, Ho CT (1986) Chromatographic analyses of gingerol compounds in ginger (Zingiber officinale Roscoe) extracted by liquid carbon dioxide. J Chromatogr A 360:163–173. https://doi.org/10.1016/S0021-9673(00)91659-5

Article  CAS  Google Scholar 

International Conference on Harmonization, ICH Q2 (R2) (2024) Validation of analytical text and methodology. Geneva, pp 1–17. https://www.ich.org/page/ich-guidlins

El-Malla SF, Hamza AA, Elagamy SH (2024) Thin-layer chromatography‒densitometric method for the simultaneous determination of paracetamol and methocarbamol in their dosage form. JPC-J Planar Chromat 37:547–556. https://doi.org/10.1007/s00764-024-00326-8

Article  CAS  Google Scholar 

Panchal PM, Shah DA (2025) Simultaneous quantification of quercetin and solasodine biomarkers from Solanum nigrum Linn. berry extract using a validated high-performance thin-layer chromatography-densitometric method. JPC-J Planar Chromat 38:553–563. https://doi.org/10.1007/s00764-025-00371-x

Article  CAS