Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures

Büchs J, Maier U, Milbradt C, Zoels B. Power consumption in shaking flasks on rotary shaking machines: I. Power consumption measurement in unbaffled flasks at low liquid viscosity. Biotechnol Bioeng. 2000;68(6):589–93.

Article  Google Scholar 

Büchs J, Maier U, Milbradt C, Zoels B. Power consumption in shaking flasks on rotary shaking machines: II. Nondimensional description of specific power consumption and flow regimes in unbaffled flasks at elevated liquid viscosity. Biotechnol Bioeng. 2000;68(6):594–601.

Article  Google Scholar 

Büchs J, Lotter S, Milbradt C. Out-of-phase operating conditions, a hitherto unknown phenomenon in shaking bioreactors. Biochem Eng J. 2001;7(2):135–41.

Article  Google Scholar 

Maier U, Büchs J. Characterisation of the gas-liquid mass transfer in shaking bioreactors. Biochem Eng J. 2001;7:99–106.

Article  Google Scholar 

Maier U, Losen M, Büchs J. Advances in Understanding and modeling the gas-liquid mass transfer in shake flasks. Biochem Eng J. 2004;17:155–67.

Article  Google Scholar 

Klöckner W, Büchs J. Advances in shaking technologies. Trends Biotechnol. 2012;30(6):307–14.

Article  Google Scholar 

Kumar S, Wittmann C, Heinzle E, Review. Minibioreactors Biotechnol Lett. 2004;26(1):1–10.

Article  Google Scholar 

Anderlei T, Büchs J. Device for sterile online measurement of the oxygen transfer rate in shaking flasks. Biochem Eng J. 2001;7:157–62.

Article  Google Scholar 

Anderlei T, Zang W, Papaspyrou M, Büchs J. Online respiration activity measurement (OTR, CTR, RQ) in shake flasks. In: Biochemical Engineering Journal. Elsevier; 2004. pp. 187–94.

Flitsch D, Ladner T, Lukacs M, Büchs J. Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles. Microb Cell Fact. 2016;15(1):45.

Article  Google Scholar 

Bracharz F, Redai V, Bach K, Qoura F, Brück T. The effects of TORC signal interference on lipogenesis in the oleaginous yeast Trichosporon oleaginosus. BMC Biotechnol. 2017;17(1):27.

Article  Google Scholar 

Gottardi M, Knudsen JD, Prado L, Oreb M, Branduardi P, Boles E. De Novo biosynthesis of trans-cinnamic acid derivatives in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 2017.

Scheidle M, Klinger J, Büchs J. Combination of on-line pH and oxygen transfer rate measurement in shake flasks by fiber optical technique and respiration activity monitoring system (RAMOS). Sensors. 2007;7(12):3472–80.

Article  Google Scholar 

Giese H, Azizan A, Kümmel A, Liao A, Peter CP, Fonseca JA, et al. Liquid films on shake flask walls explain increasing maximum oxygen transfer capacities with elevating viscosity. Biotechnol Bioeng. 2014;111(2):295–308.

Article  Google Scholar 

Giese H, Klöckner W, Peña C, Galindo E, Lotter S, Wetzel K, et al. Effective shear rates in shake flasks. Chem Eng Sci. 2014;118:102–13.

Article  Google Scholar 

Tesche S, Rösemeier-Scheumann R, Lohr J, Hanke R, Büchs J, Krull R. Salt-enhanced cultivation as a morphology engineering tool for filamentous actinomycetes: increased production of labyrinthopeptin A1 in Actinomadura Namibiensis. Eng Life Sci. 2019;19(11):781–94.

Article  Google Scholar 

Halmschlag B, Völker F, Hanke R, Putri SP, Fukusaki E, Büchs J et al. Metabolic engineering of B. subtilis 168 for increased precursor supply and poly-γ-glutamic acid production. Front Food Sci Technol. 2023;3.

Halmschlag B, Hoffmann K, Hanke R, Putri SP, Fukusaki E, Büchs J et al. Comparison of isomerase and Weimberg pathway for γ-PGA production from xylose by engineered Bacillus subtilis. Front Bioeng Biotechnol. 2020;7.

Peña C, Peter CP, Büchs J, Galindo E. Evolution of the specific power consumption and oxygen transfer rate in alginate-producing cultures of Azotobacter vinelandii conducted in shake flasks. Biochem Eng J. 2007;36(2):73–80.

Article  Google Scholar 

García-Ochoa F, Santos VE, Casas JA, Gómez E. Xanthan gum: production, recovery, and properties. Biotechnol Adv. 2000;18(7):549–79.

Article  Google Scholar 

Peña C, Galindo E, Büchs J. The viscosifying power, degree of acetylation and molecular mass of the alginate produced by Azotobacter vinelandii in shake flasks are determined by the oxygen transfer rate. Process Biochem. 2011;46(1):290–7.

Article  Google Scholar 

Sparviero S, Dicke MD, Rosch TM, Castillo T, Salgado-Lugo H, Galindo E et al. Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046. Microb Cell Fact. 2023;22(1):1–24. Available from: https://doi.org/10.1186/s12934-023-02112-3

Wilming A, Begemann J, Kuhne S, Regestein L, Bongaerts J, Evers S et al. Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations. Biochem Eng J. 2013;73:29–37. Available from: https://doi.org/10.1016/j.bej.2013.01.008

Regestein née Meissner L, Arndt J, Palmen TG, Jestel T, Mitsunaga H, Fukusaki E, et al. Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks. J Biol Eng. 2017;11(1):1–16.

Article  Google Scholar 

Alvarez-Yela AC, Chiquiza-Montaño LN, Hoyos R, Orozco-Sánchez F. Rheology and mixing analysis of plant cell cultures (Azadirachta indica, Borojoa patinoi and Thevetia peruviana) in shake flasks. Biochem Eng J. 2016;114:18–25.

Article  Google Scholar 

Rodrı́guez-Monroy M, Galindo E. Broth rheology, growth and metabolite production of Beta vulgaris suspension culture: a comparative study between cultures grown in shake flasks and in a stirred tank. Enzyme Microb Technol. 1999;24(10):687–93.

Article  Google Scholar 

Bliatsiou C, Schrinner K, Waldherr P, Tesche S, Böhm L, Kraume M et al. Rheological characteristics of filamentous cultivation broths and suitable model fluids. Biochem Eng J. 2020;163(August).

Böl M, Schrinner K, Tesche S, Krull R. Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems - a critical review. Eng Life Sci. 2021;21(3–4):51–67.

Article  Google Scholar 

Wucherpfennig T, Hestler T, Krull R. Morphology engineering - osmolality and its effect on Aspergillus niger morphology and productivity. Microb Cell Fact. 2011;10(58). Available from: http://www.microbialcellfactories.com/content/10/1/58

Finger M, Sentek F, Hartmann L, Palacio-Barrera AM, Schlembach I, Rosenbaum MA, et al. Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high-throughput online monitoring. Eng Life Sci. 2023;23(1):1–10.

Article  Google Scholar 

Goudar CT, Strevett KA, Shah SN. Influence of microbial concentration on the rheology of non-Newtonian fermentation broths. Appl Microbiol Biotechnol. 1999;51(3):310–5.

Article  Google Scholar 

Karsheva M, Hristov J, Penchev I, Lossev V. Rheological behavior of fermentation broths in antibiotic industry. Appl Biochem Biotechnol. 1997;68(3):187–206.

Article  Google Scholar 

Vanags JJ, Viesturs UE, Priede MA. Studies of the mixing character and flow distribution in mycelial fermentation broths. Acta Biotechnol. 1995;15(4):355–66.

Article  Google Scholar 

Dhillon GS, Brar SK, Kaur S, Verma M. Rheological studies during submerged citric acid fermentation by Aspergillus Niger in stirred fermentor using Apple pomace ultrafiltration sludge. Food Bioprocess Technol. 2013;6(5):1240–50.

Article  Google Scholar 

Domingues FC, Queiroz JA, Cabral JM, Fonseca LP. The influence of culture conditions on mycelial structure and cellulase production by Trichoderma Reesei Rut C-30. Enzyme Microb Technol. 2000;26(5–6):394–401.

Article  Google Scholar 

Schlembach I, Hosseinpour Tehrani H, Blank LM, Büchs J, Wierckx N, Regestein L et al. Consolidated bioprocessing of cellulose to itaconic acid by a co-culture of Trichoderma reesei and Ustilago maydis. Biotechnol Biofuels. 2020;13(1):1–18. Available from: https://doi.org/10.1186/s13068-020-01835-4

Lima PC, Karimian P, Johnston E, Hartley CJ. The use of trichoderma spp. For the bioconversion of Agro-Industrial waste biomass via fermentation: A review. Fermentation. 2024;10(9):442.

Article  Google Scholar 

Simon L, Caye-Vaugien C, Bouchonneau M. Relation between Pullulan production, morphological state and growth conditions in Aureobasidium Pullulans: new observations. J Gen Microbiol. 1993;139(5):979–85.

Article  Google Scholar 

Rau U, Brandt C. Oxygen controlled batch cultivations of Schizophyllum commune for enhanced production of branched β-1,3-glucans. Bioprocess Eng. 1994;11(4):161.

Article  Google Scholar 

Tan R-K, Eberhard W, Büchs J. Measurement and characterization of mixing time in shake flasks. Chem Eng Sci. 2011;66(3):440–7.

Article  Google Scholar 

Azizan A, Büchs J. Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique. J Biol Eng. 2017;11(1):1–9.

Article  Google Scholar 

Dinter C, Gumprecht A, Menze MA, Azizan A, Niehoff PJ, Hansen S et al. Validation of computational fluid dynamics of shake flask experiments at moderate viscosity by liquid distributions and volumetric power inputs. Sci Rep. 2024;14(1):1–17. Available from: https://doi.org/10.1038/s41598-024-53980-7

Humphrey A. Shake flask to fermentor: what have we learned? Biotechnol Prog. 1998;14(1):3–7.

Article  Google Scholar 

McNeil B, Harvey LM. Viscous fermentation products. Crit Rev Biotechnol. 1993;13(4):275–304.

Article  Google Scholar 

Büchs J. Introduction to advantages and problems of shaken cultures. Biochem Eng J. 2001;7(2):91–8.

Article  Google Scholar 

Converti A, Zilli M, Arni S, Di Felice R, Del Borghi M. Estimation of viscosity of highly viscous fermentation media containing one or more solutes. Biochem Eng J. 1999;4(1):81–5.

Article  Google Scholar 

Blanch HW, Bhavaraju SM. Non-Newtonian fermentation broths: rheology and mass transfer. Biotechnol Bioeng. 1976;18(6):745–90.

Article  Google Scholar 

Doran PM. In: Doran PM, editor. Bioprocess engineering erinciples, second edition. London: Academic; 2012. p. 928.

Google Scholar 

Wunderlich M, Trampnau PP, Lopes EF, Büchs J, Regestein L. Online in situ viscosity determination in stirred tank reactors by measurement of the heat transfer capacity. Chem Eng Sci. 2016;152:116–26.

Article  Google Scholar 

Schelden M, Lima W, Doerr EW, Wunderlich M, Rehmann L, Büchs J, et al. Online measurement of viscosity for biological systems in stirred tank bioreactors. Biotechnol Bioeng. 2017;114(5):990–7.

Article  Google Scholar 

Zimmermann HF, Anderlei T, Büchs J, Binder M. Oxygen limitation is a pitfall during screening for industrial strains. Appl Microbiol Biotechnol. 2006;72(6):1157–60.

Comments (0)

No login
gif