Murine model of antibiotic-associated Staphylococcus aureus gastrointestinal infections (SAGII) and colonization

Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015;28(3):603–61.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Oliveira D, Borges A, Simões M, MDPI AG. Staphylococcus aureus toxins and their molecular activity in infectious diseases. Toxins (Basel). 2018. https://doi.org/10.3390/toxins10060252.

Article  PubMed  PubMed Central  Google Scholar 

Missiakas DM, Schneewind O. Growth and laboratory maintenance of Staphylococcus aureus. Curr Protoc Microbiol. 2013; Chapter9:Unit-9C.1 .

Wang JL, Chen SY, Wang JT, et al. Comparison of both clinical features and mortality risk associated with bacteremia due to community-acquired methicillin-resistant Staphylococcus aureus and methicillin-susceptible S. aureus. Clin Infect Dis. 2008;46(6):799–806.

Article  PubMed  Google Scholar 

Ahmad-Mansour N, Loubet P, Pouget C, Toxins et al. (Basel). MDPI; 2021.

Foster TJ, Geoghegan JA, Ganesh VK, Höök M. Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nat Rev Microbiol. 2014 ;12(1). pp. 49–62.

Berube BJ, Wardenburg JB. Staphylococcus aureus α-toxin: nearly a century of intrigue. Toxins (Basel). 2013. https://doi.org/10.3390/toxins5061140.

Article  PubMed  Google Scholar 

Thammavongsa V, Kim HK, Missiakas D, Schneewind O. Staphylococcal manipulation of host immune responses. Nat Rev Microbiol Nat Publishing Group. 2015 ; 13(9). 529–43.

Gehrke AKE, Giai C, Gómez MI, Multidisciplinary Digital Publishing Institute (MDPI). Staphylococcus aureus adaptation to the skin in health and persistent/recurrent infections. Antibiotics. 2023. https://doi.org/10.3390/antibiotics12101520.

Article  PubMed  PubMed Central  Google Scholar 

Vozza EG, Kelly AM, Daly CM et al. Type 1 interferons promote Staphylococcus aureus nasal colonization by inducing phagocyte apoptosis. Cell Death Discov [Internet]. 2024; 10(1):403. Available from: https://www.nature.com/articles/s41420-024-02173-2

Baur S, Rautenberg M, Faulstich M et al. A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization. PLoS Pathog Public Libr Sci. 2014. 10(5):e1004089.

Otto M. Staphylococcus colonization of the skin and antimicrobial peptides. Expert Rev Dermatol. 2010 ;5(2):183-195. https://doi.org/10.1586/edm.10.6

Article  PubMed  PubMed Central  Google Scholar 

Verhoeven PO, Gagnaire J, Botelho-Nevers E, et al. Detection and clinical relevance of Staphylococcus aureus nasal carriage: an update. Expert Rev Anti-Infect Ther. 2014 ;12(1):75-89. https://doi.org/10.1586/14787210.2014.859985

Article  PubMed  Google Scholar 

Parras F, Del M, Guerrero C et al. Comparative Study of Mupirocin and Oral Co-Trimoxazole plus topical fusidic acid in eradication of nasal carriage of methicillin-resistant staphylococcus aureus [Internet]. Antimicrob Agents Chemother. 1995. Available from: https://journals.asm.org/journal/aac

Gagnaire J, Verhoeven PO, Grattard F, et al. Epidemiology and clinical relevance of Staphylococcus aureus intestinal carriage: a systematic review and meta-analysis. Expert Rev Anti Infect Ther. Taylor and Francis Ltd; 2017. pp. 767–85.

Piewngam P, Otto M. Staphylococcus aureus colonisation and strategies for decolonisation. Lancet microbe. Elsevier Ltd; 2024. pp. e606–18.

Larcombe S, Jiang JH, Hutton ML, Abud HE, Peleg AY, Lyras D. A mouse model of Staphylococcus aureus small intestinal infection. J Med Microbiol. 2020;69(2):290–7.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Chen X, Katchar K, Goldsmith JD, et al. A mouse model of clostridium difficile-associated disease. Gastroenterology. Volume 135. W.B. Saunders; 2008. pp. 1984–92. 6.

Barbut F. Managing antibiotic associated diarrhoea. BMJ BMJ. 2002;324(7350):1345–6.

Article  PubMed  Google Scholar 

Acton DS, Tempelmans Plat-Sinnige MJ, Wamel W, Van, Groot N, De, Belkum A, Van. Intestinal carriage of Staphylococcus aureus: how does its frequency compare with that of nasal carriage and what is its clinical impact? Eur J Clin Microbiol Infect Dis. 2009. pp. 115–27.

Allen Editor IC. Mouse models of innate immunity methods and protocols methods in molecular biology 1031 [Internet]. Available from: http://www.springer.com/series/7651

Rauch S, DeDent AC, Kim HK, Wardenburg JB, Missiakas DM, Schneewind O. Abscess formation and alpha-hemolysin induced toxicity in a mouse model of Staphylococcus aureus peritoneal infection. Infect Immun. 2012;80(10):3721–32.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Liang H, Wang Y, Liu F, et al. The application of rat models in Staphylococcus aureus infections. Pathogens. Multidisciplinary Digital Publishing Institute (MDPI); 2024.

Salgado BAB, Waters EM, Moran JC, Kadioglu A, Horsburgh MJ. Selection of Staphylococcus aureus in a murine nasopharyngeal colonization model. Front Cell Infect Microbiol. 2022 ;12:874138. https://doi.org/10.3389/fcimb.2022.874138

Article  PubMed  PubMed Central  Google Scholar 

Misawa Y, Kelley KA, Wang X, et al. Staphylococcus aureus colonization of the mouse gastrointestinal tract is modulated by wall teichoic acid, capsule, and surface proteins. PLoS Pathog. 2015 ;11(7):e1005061. https://doi.org/10.1371/journal.ppat.1005061

Article  PubMed  PubMed Central  Google Scholar 

da Silva JG, Boechat JPC, Silva BDJ, Müller R, Senna JPM. Monitoring Staphylococcus aureus nasal colonization murine model using a bioluminescent methicillin-resistant S. aureus (MRSA). Lab Anim. Volume 58. SAGE Publications Ltd; 2024. pp. 231–9. 3.

Gries DM, Pultz NJ, Donskey CJ. Stokes cleveland l. growth in cecal mucus facilitates colonization of the mouse intestinal tract by methicillin-resistant staphylococcus aureus [Internet]. J Infect Dis. 2005. Available from: https://academic.oup.com/jid/article/192/9/1621/836227

Yoshida Y. Methicillin-resistant Staphylococcus aureus proliferation in the rat gut is influenced by gastric acid Inhibition and the administration of antibiotics. Surg. Today . 1999;29(4):327-37

Liu G, Pang B, Li N, et al. Therapeutic effect of: Lactobacillus rhamnosus SHA113 on intestinal infection by multi-drug-resistant Staphylococcus aureus and its underlying mechanisms. Volume 11. Food Funct. Royal Society of Chemistry; 2020. pp. 6226–39. 7.

Nakamura YAYKT. A mouse model for postoperative fatal enteritis due to Staphylococcus infection. J Surg Res. 2001;96(1):35–43.

Article  PubMed  CAS  Google Scholar 

Iwata K, Doi A, Fukuchi T, et al. A systematic review for pursuing the presence of antibiotic associated enterocolitis caused by methicillin resistant Staphylococcus aureus. BMC Infect Dis. 2014 ; 14:247. https://doi.org/10.1186/1471-2334-14-247

Article  PubMed  PubMed Central  Google Scholar 

Buffie CG, PEG. Microbiota-mediated colonization resistance against intestinal pathogens. Nat Rev Immunol. (2013). 13, 790–801.

Kennedy EA, King KY, Baldridge MT. Mouse microbiota models: comparing germ-free mice and antibiotics treatment as tools for modifying gut bacteria. Front Physiol Front Media S A; 2018; 9 :1534

Howerton A, Patra M, Abel-Santos E. A new strategy for the prevention of clostridium difficile infection. J Infect Dis. 2013;207(10):1498–504.

Article  PubMed  CAS  Google Scholar 

Bhute SS, Mefferd CC, Phan JR, et al. A high-carbohydrate diet prolongs dysbiosis and clostridioides difficile carriage and increases delayed mortality in a hamster model of infection. Volume 10. Microbiol Spectr. American Society for Microbiology; 2022. 4.

Wahlström A, Sayin SI, Marschall HU, Bäckhed F, Cell Press. Intestinal crosstalk between bile acids and microbiota and its impact on host metabolism. Cell Metab. 2016. https://doi.org/10.1016/j.cmet.2016.05.005.

Article  PubMed  Google Scholar 

Ridlon JM, Kang DJ, Hylemon PB, Bajaj JS, Lippincott Williams and Wilkins. Bile acids and the gut microbiome. Curr Opin Gastroenterol. 2014. https://doi.org/10.1097/MOG.0000000000000057.

Article  PubMed  PubMed Central  Google Scholar 

Desai MS, Seekatz AM, Koropatkin NM, et al. A dietary fiber-deprived gut microbiota degrades the colonic mucus barrier and enhances pathogen susceptibility. Cell Cell Press. 2016;167(5):1339–e135321.

CAS  Google Scholar 

Venegas DP, La Fuente MK, De, Landskron G, et al. Short chain fatty acids (SCFAs)mediated gut epithelial and immune regulation and its relevance for inflammatory bowel diseases. Front Immunol. Frontiers Media S.A.; 2019.

Fan J, Wang L, Yang T, et al. Comparative analysis of gut microbiota in incident and prevalent peritoneal Dialysis patients with peritoneal fibrosis, correlations with peritoneal equilibration test data in the peritoneal fibrosis cohort. John Wiley and Sons Inc: Therapeutic Apheresis and Dialysis; 2024.

Book  Google Scholar 

Mojgani N, Ashique S, Moradi M, et al. Gut microbiota and postbiotic metabolites: biotic intervention for enhancing vaccine responses and personalized medicine for disease prevention. Probiotics Antimicrob Proteins. Springer; 2025.

Li L, Li T, Liang X, et al. A decrease in flavonifractor plautii and its product, phytosphingosine, predisposes individuals with phlegm-dampness constitution to metabolic disorders. Cell Discov. 2025 ;11(1):25 . https://doi.org/10.1038/s41421-025-00789-x

Article  PubMed  PubMed Central  Google Scholar 

Lejeune A, Zhou C, Ercelen D et al. Sex-dependent gastrointestinal colonization resistance to MRSA is microbiota and Th17 dependent [Internet]. 2025. Available from: https://elifesciences.org/reviewed-preprints/101606v2

Selvaraj V, Alsamman MA. Antibiotic-associated diarrhea beyond c. difficile: A scoping review. Journal of Brown Hospital Medicine. Department of Medicine, Warren Alpert Medical School at Brown University; 2022; 2(1).

Nair D, Memmi G, Hernandez D, et al. Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. J Bacteriol. 2011;193(9):2332–5.

Article  PubMed  PubMed Central  CAS  Google Scholar 

Kaito C, Kurokawa K, Matsumoto Y, et al. Silkworm pathogenic bacteria infection model for identification of novel virulence genes. Mol Microbiol. 2005;56(4):934–44.

Article  PubMed  CAS  Google Scholar 

Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clin Microbiol Rev. 2014;27(4):870–926.

Article  PubMed  PubMed Central  Google Scholar 

Gordon RJ, Lowy FD. Pathogenesis of methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis. 2008 ; 46(Suppl 5):S350-9. https://doi.org/10.1086/533591

Article  PubMed  Google Scholar 

Diep BA, Gill SR, Chang RF, et al. Complete genome sequence of USA300, an epidemic clone of community-acquired methicillin-resistant Staphylococcus aureus. Lancet. 2006;367(9512):731–9.

Article  PubMed  CAS  Google Scholar 

Hurley BW, Cuong; NC. The Spectrum of Pseudomembranous Enterocolitis and Antibiotic-Associated Diarrhea.

Cho JS, Pietras EM, Garcia NC, et al. IL-17 is essential for host defense against cutaneous Staphylococcus aureus infection in mice. J Clin Invest. 2010;120(5):1762–73.

Article  PubMed 

Comments (0)

No login
gif