Weber JA, Baxter DH, Zhang S, Huang DY, Huang KH, Lee MJ, et al. The MicroRNA spectrum in 12 body fluids. Clin Chem. 2010;56(11):1733–41. https://doi.org/10.1373/clinchem.2010.147405.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang D, Farhana A. Biochemistry, RNA Structure. In: StatPearls. Treasure Island (FL): StatPearls Publishing. 2023.

Witkowska-Zimny M, Kaminska-El-Hassan E. Cells of human breast milk. Cell Mol Biol Lett. 2017;22:1–11.

Article  Google Scholar 

Cai C, Eck P, Friel JK. Gene expression profiles suggest iron transport pathway in the lactating human epithelial cell. J Pediatr Gastroenterol Nutr. 2017;64(3):460–4. https://doi.org/10.1097/MPG.0000000000001303.

Article  CAS  PubMed  Google Scholar 

Groneberg DA, Döring F, Theis S, Nickolaus M, Fischer A, Daniel H. Peptide transport in the mammary gland: expression and distribution of PEPT2 mRNA and protein. Am J Physiology-Endocrinology Metabolism. 2002;282(5):E1172–9.

Article  CAS  Google Scholar 

Tingo L, Ahlberg E, Johansson L, Pedersen SA, Chawla K, Saetrom P, et al. Non-Coding RNAs in human breast milk: A systematic review. Front Immunol. 2021;12:725323. https://doi.org/10.3389/fimmu.2021.725323.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Munch EM, Harris RA, Mohammad M, Benham AL, Pejerrey SM, Showalter L, et al. Transcriptome profiling of MicroRNA by Next-Gen deep sequencing reveals known and novel MiRNA species in the lipid fraction of human breast milk. PLoS ONE. 2013;8(2):e50564.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kosaka N, Izumi H, Sekine K, Ochiya T. MicroRNA as a new immune-regulatory agent in breast milk. Silence. 2010;1:1–8.

Article  Google Scholar 

Alsaweed M, Lai CT, Hartmann PE, Geddes DT, Kakulas F. Human milk MiRNAs primarily originate from the mammary gland resulting in unique MiRNA profiles of fractionated milk. Sci Rep. 2016;6(1):20680.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Munch EM, Harris RA, Mohammad M, Benham AL, Pejerrey SM, Showalter L, et al. Transcriptome profiling of MicroRNA by Next-Gen deep sequencing reveals known and novel MiRNA species in the lipid fraction of human breast milk. PLoS ONE. 2013;8(2):e50564. https://doi.org/10.1371/journal.pone.0050564.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Alsaweed M, Hartmann PE, Geddes DT, Kakulas F. MicroRNAs in breastmilk and the lactating breast: potential immunoprotectors and developmental regulators for the infant and the mother. Int J Environ Res Public Health. 2015;12(11):13981–4020. https://doi.org/10.3390/ijerph121113981.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Freiría-Martínez L, Iglesias-Martínez-Almeida M, Rodríguez-Jamardo C, Rivera-Baltanás T, Comís-Tuche M, Rodrígues-Amorím D, et al. Human breast milk microRNAs, potential players in the regulation of nervous system. Nutrients. 2023;15(14):3284.

Article  PubMed  PubMed Central  Google Scholar 

Kim SY, Yi DY. Components of human breast milk: from macronutrient to Microbiome and MicroRNA. Clin Exp Pediatr. 2020;63(8):301–9. https://doi.org/10.3345/cep.2020.00059.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yeruva L, Mulakala BK, Rajasundaram D, Gonzalez S, Cabrera-Rubio R, Martinez-Costa C, et al. Human milk MiRNAs associate to maternal dietary nutrients, milk microbiota, infant gut microbiota and growth. Clin Nutr. 2023;42(12):2528–39. https://doi.org/10.1016/j.clnu.2023.10.011.

Article  CAS  PubMed  Google Scholar 

Kaeffer B. Human breast milk mirnas: their diversity and potential for preventive strategies in nutritional therapy. Int J Mol Sci. 2023;24(22):16106.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Melnik BC, Kakulas F, Geddes DT, Hartmann PE, John SM, Carrera-Bastos P, et al. Milk mirnas: simple nutrients or systemic functional regulators? Nutr Metabolism. 2016;13:1–5.

Article  Google Scholar 

Raymond F, Lefebvre G, Texari L, Pruvost S, Metairon S, Cottenet G, et al. Longitudinal human milk MiRNA composition over the first 3 mo of lactation in a cohort of healthy mothers delivering term infants. J Nutr. 2022;152(1):94–106.

Article  CAS  PubMed  Google Scholar 

Wieser M, Burger S, Ertl R, Kummer S, Stargardt M, Walter I. Example for process validation in biobanking: fit for purpose testing of a cryopreservation method without isopentane. Front Mol Biosci. 2022;9:876670. https://doi.org/10.3389/fmolb.2022.876670.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shabihkhani M, Lucey GM, Wei B, Mareninov S, Lou JJ, Vinters HV, et al. The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings. Clin Biochem. 2014;47(4–5):258–66. https://doi.org/10.1016/j.clinbiochem.2014.01.002.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Alsaweed M, Hepworth AR, Lefevre C, Hartmann PE, Geddes DT, Hassiotou F. Human milk MicroRNA and total RNA differ depending on milk fractionation. J Cell Biochem. 2015;116(10):2397–407. https://doi.org/10.1002/jcb.25207.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ahlberg E, Jenmalm MC, Tingo L. Evaluation of five column-based isolation kits and their ability to extract MiRNA from human milk. J Cell Mol Med. 2021;25(16):7973–9. https://doi.org/10.1111/jcmm.16726.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sohel MH. Extracellular/circulating micrornas: release mechanisms, functions and challenges. Achievements Life Sci. 2016;10(2):175–86.

Article  Google Scholar 

Zhang Y, Liu Y, Liu H, Tang WH. Exosomes: biogenesis, biologic function and clinical potential. Cell Bioscience. 2019;9:1–18.

Article  Google Scholar 

Alsaweed M, Lai CT, Hartmann PE, Geddes DT, Kakulas F. Human milk cells contain numerous MiRNAs that May change with milk removal and regulate multiple physiological processes. Int J Mol Sci. 2016;17(6):956.

Article  PubMed  PubMed Central  Google Scholar 

Zamanillo R, Sánchez J, Serra F, Palou A. Breast milk supply of MicroRNA associated with leptin and adiponectin is affected by maternal overweight/obesity and influences infancy BMI. Nutrients. 2019;11(11):2589.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhou Q, Li M, Wang X, Li Q, Wang T, Zhu Q, et al. Immune-related MicroRNAs are abundant in breast milk exosomes. Int J Biol Sci. 2011;8(1):118.

Article  PubMed  PubMed Central  Google Scholar 

Wang H, Wu D, Sukreet S, Delaney A, Belfort MB, Zempleni J. Quantitation of exosomes and their MicroRNA cargos in frozen human milk. JPGN Rep. 2022;3(1):e172. https://doi.org/10.1097/pg9.0000000000000172.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Leiferman A, Shu J, Upadhyaya B, Cui J, Zempleni J. Storage of extracellular vesicles in human milk, and MicroRNA profiles in human milk exosomes and infant formulas. J Pediatr Gastroenterol Nutr. 2019;69(2):235–8. https://doi.org/10.1097/MPG.0000000000002363.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Andreasson A, Kiss NB, Juhlin CC, Hoog A. Long-term storage of endocrine tissues at – 80 degrees C does not adversely affect RNA quality or overall histomorphology. Biopreserv Biobank. 2013;11(6):366–70. https://doi.org/10.1089/bio.2013.0038.

Article  CAS  PubMed  PubMed Central