Dalamaga M. Clinical metabolomics: useful insights, perspectives and challenges. Metab Open. 2024;22:100290.

Article  CAS  Google Scholar 

Abdel Rahman AM. The advanced technology and clinical application in metabolomics. In: Abdel Rahman AM, editor. Clinical Metabolomics Applications in Genetic Diseases. Singapore: Springer; 2023. pp. 1–15.

Chapter  Google Scholar 

Skogvold HB, Sand ES, Elgstøen KBP. Global etabolomics using LC-MS for clinical applications. In: Giera M, Sánchez-López E, editors. Clinical Metabolomics. Methods in Molecular Biology. Volume 2855. New York, NY: Humana; 2025. pp. 23–39.

Chapter  Google Scholar 

Dunn WB, Broadhurst D, Begley P, Zelena E, Francis-Mcintyre S, Anderson N, Brown M, Knowles JD, Halsall A, Haselden JN, Nicholls AW, Wilson ID, Kell DB, Goodacre R. Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nat Protoc. 2011;6(7):1060–83.

Article  CAS  PubMed  Google Scholar 

Chen D, Zhao S, Li L, Li L. Controlling pre-analytical process in human serum/plasma metabolomics. TrAC - Trends Anal Chem. 2023;169:117364.

Article  CAS  Google Scholar 

González-Domínguez R, González-Domínguez Á, Sayago A, Fernández-Recamales Á. Recommendations and best practices for standardizing the pre-analytical processing of blood and urine samples in metabolomics. Metabolites. 2020;10:1–18.

Article  Google Scholar 

Yin P, Lehmann R, Xu G. Effects of pre-analytical processes on blood samples used in metabolomics studies. Anal Bioanal Chem. 2015;407:4879–92.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen D, Han W, Huan T, Li L, Li L. Effects of freeze-thaw cycles of blood samples on high-coverage quantitative metabolomics. Anal Chem. 2020;92(13):9265–72.

Article  CAS  PubMed  Google Scholar 

Garwolińska D, Kot-Wasik A, Hewelt-Belka W. Pre-analytical aspects in metabolomics of human biofluids - sample collection, handling, transport, and storage. Mol Omics. 2022;19:95–104.

Article  Google Scholar 

Torell F, Bennett K, Rännar S, Lundstedt-Enkel K, Lundstedt T, Trygg J. The effects of thawing on the plasma metabolome: evaluating differences between thawed plasma and multi-organ samples. Metabolomics. 2017;13(6):66.

Article  PubMed  PubMed Central  Google Scholar 

McClain KM, Moore SC, Sampson JN, Henderson TR, Gebauer SK, Newman JW, Ross S, Pedersen TL, Baer DJ, Zanetti KA. Preanalytical sample handling conditions and their effects on the human serum metabolome in epidemiologic studies. Am J Epidemiol. 2021;190(3):459–67.

Article  PubMed  PubMed Central  Google Scholar 

Anton G, Wilson R, Yu ZH, Prehn C, Zukunft S, Adamski J, Heier M, Meisinger C, Römisch-Margl W, Wang-Sattler R, Hveem K, Wolfenbuttel B, Peters A, Kastenmüller G, Waldenberger M. Pre-analytical sample quality: Metabolite ratios as an intrinsic marker for prolonged room temperature exposure of serum samples. PLoS ONE. 2015;10(3):e0121495.

Article  PubMed  PubMed Central  Google Scholar 

Buchanan JL, Tormes Vaquerano J, Taylor EB. Isolated effects of plasma freezing versus thawing on metabolite stability. Metabolites. 2022;12(11):1098.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pizarro C, Arenzana-Rámila I, Pérez-del-Notario N, Pérez-Matute P, González-Sáiz JM. Thawing as a critical pre-analytical step in the lipidomic profiling of plasma samples: New standardized protocol. Anal Chim Acta. 2016;912:1–9.

Article  CAS  PubMed  Google Scholar 

Mittelstrass K, Ried JS, Yu Z, Krumsiek J, Gieger C, Prehn C, Roemisch-Margl W, Polonikov A, Peters A, Theis FJ, Meitinger T, Kronenberg F, Weidinger S, Wichmann HE, Suhre K, Wang-Sattler R, Adamski J, Illig T. Discovery of sexual dimorphisms in metabolic and genetic biomarkers. PLoS Genet. 2011;7(8):e1002215.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Audano M, Maldini M, De Fabiani E, Mitro N, Caruso D. Gender-related metabolomics and lipidomics: From experimental animal models to clinical evidence. J Proteom. 2018;178:82–91.

Article  CAS  Google Scholar 

Zhang Y, Zhao J, Zhao H, Lu X, Jia X, Zhao X, Xu G. Reference intervals of serum metabolites and lipids of a healthy Chinese population determined by liquid chromatography-mass spectrometry. Metabolites. 2025;15(2):106.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Costanzo M, Caterino M, Sotgiu G, Ruoppolo M, Franconi F, Campesi I. Sex differences in the human metabolome. Biol Sex Differ. 2022;13:30.

Article  PubMed  PubMed Central  Google Scholar 

Krumsiek J, Mittelstrass K, Do KT, Stückler F, Ried J, Adamski J, Peters A, Illig T, Kronenberg F, Friedrich N, Nauck M, Pietzner M, Mook-Kanamori DO, Suhre K, Gieger C, Grallert H, Theis FJ, Kastenmüller G. Gender-specific pathway differences in the human serum metabolome. Metabolomics. 2015;11(6):1815–33.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Xu Y, Wilson ID, Goodacre R. Combining clinical chemistry with metabolomics for metabolic phenotyping at population levels. Metabolomics. 2025;21(5):126.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pottel H, Hoste L, Delanaye P, Cavalier E, Martens F. Demystifying ethnic/sex differences in kidney function: Is the difference in (estimating) glomerular filtration rate or in serum creatinine concentration? Clin Chim Acta. 2012;413(19–20):1612–7.

Article  CAS  PubMed  Google Scholar 

Guo K, Li L. Differential 12C-/13C-isotope dansylation labeling and fast liquid chromatography/mass spectrometry for absolute and relative quantification of the metabolome. Anal Chem. 2009;81(10):3919–32.

Article  CAS  PubMed  Google Scholar 

Zhao S, Li H, Han W, Chan W, Li L. Metabolomic coverage of chemical-group-submetabolome analysis: Group classification and four-channel chemical isotope labeling LC-MS. Anal Chem. 2019;91(18):12108–15.

Article  CAS  PubMed  Google Scholar 

Chen D, Zhao S, Ma G, Li L, Li L. An Analytical strategy for reliable metabolome analysis of clinical leftover sera using timed aliquoting. Anal Chem. 2025;97(46):25655–63.

Article  CAS  PubMed  Google Scholar 

Zhou R, Tseng CL, Huan T, Li L, IsoMS. Automated processing of LC-MS data generated by a chemical isotope labeling metabolomics platform. Anal Chem. 2014;86(10):4675–9.

Article  CAS  PubMed  Google Scholar 

Sumner LW, Amberg A, Barrett D, Beale MH, Beger R, Daykin CA, Fan TWM, Fiehn O, Goodacre R, Griffin JL, Hankemeier T, Hardy N, Harnly J, Higashi R, Kopka J, Lane AN, Lindon JC, Marriott P, Nicholls AW, Reily MD, Thaden JJ, Viant MR. Proposed minimum reporting standards for chemical analysis: Chemical analysis working group (CAWG) metabolomics standards initiative (MSI). Metabolomics. 2007;3(3):211–21.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li L, Li R, Zhou J, Zuniga A, Stanislaus AE, Wu Y, Huan T, Zheng J, Shi Y, Wishart DS, Lin G. MyCompoundID: Using an evidence-based metabolome library for metabolite identification. Anal Chem. 2013;85(6):3401–8.

Article  CAS  PubMed  Google Scholar 

Wang Q, Hoene M, Hu C, Fritsche L, Ahrends R, Liebisch G, Ekroos K, Fritsche A, Birkenfeld AL, Liu X, Zhao X, Li Q, Su B, Peter A, Xu G, Lehmann R. Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies. J Lipid Res. 2023;64(6):100378.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Long NP, Park S, Anh NH, Kim SJ, Kim HM, Yoon SJ, Lim J, Kwon SW. Advances in liquid chromatography–mass spectrometry-based lipidomics: A look ahead. J Anal Test. 2020;4:183–97.

Article  Google Scholar 

Han X, Gross RW. The foundations and development of lipidomics. J Lipid Res. 2022;63(2):100164.

Article  CAS  PubMed  Google Scholar