Scott RT Jr, Upham KM, Forman EJ, Hong KH, Scott KL, Taylor D, Tao X, Treff NR. Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial. Fertil Steril. 2013;100(3):697–703. https://doi.org/10.1016/j.fertnstert.2013.04.035.
Tiegs AW, Tao X, Zhan Y, Whitehead C, Kim J, Hanson B, Osman E, Kim TJ, Patounakis G, Gutmann J, Castelbaum A, Seli E, Jalas C, Scott RT Jr. A multicenter, prospective, blinded, nonselection study evaluating the predictive value of an aneuploid diagnosis using a targeted next-generation sequencing-based preimplantation genetic testing for aneuploidy assay and impact of biopsy. Fertil Steril. 2021;115(3):627–37. https://doi.org/10.1016/j.fertnstert.2020.07.052.
Zhang S, Luo K, Cheng D, Tan Y, Lu C, He H, Gu Y, Lu G, Gong F, Lin G. Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality. Fertil Steril. 2016;105(5):1222-1227.e4. https://doi.org/10.1016/j.fertnstert.2016.01.011.
Munné S, Kaplan B, Frattarelli JL, Child T, Nakhuda G, Shamma FN, Silverberg K, Kalista T, Handyside AH, Katz-Jaffe M, Wells D, Gordon T, Stock-Myer S, Willman S, STAR Study Group. Preimplantation genetic testing for aneuploidy versus morphology as selection criteria for single frozen-thawed embryo transfer in good-prognosis patients: a multicenter randomized clinical trial. Fertil Steril. 2019;112(6):1071-1079.e7. https://doi.org/10.1016/j.fertnstert.2019.07.1346.
Article CAS PubMed Google Scholar
Ozgur K, Berkkanoglu M, Bulut H, et al. Single best euploid versus single best unknown-ploidy blastocyst frozen embryo transfers: a randomized controlled trial. J Assist Reprod Genet. 2019;36:629–36. https://doi.org/10.1007/s10815-018-01399-1.
Article PubMed PubMed Central Google Scholar
Zhang WY, von Versen-Höynck F, Kapphahn KI, Fleischmann RR, Zhao Q, Baker VL. Maternal and neonatal outcomes associated with trophectoderm biopsy. Fertil Steril. 2019;112(2):283-290.e2. https://doi.org/10.1016/j.fertnstert.2019.03.033.
Article PubMed PubMed Central Google Scholar
Lu MM, Wen YX, Liu YL, Ding CH, Zhou CQ, Xu YW. Trophectoderm biopsy reduces the level of serum β-human chorionic gonadotropin in early pregnancy. Fertil Steril. 2020;114(4):801–8. https://doi.org/10.1016/j.fertnstert.2020.05.015.
Article CAS PubMed Google Scholar
Zhao Y, Zhao Y, Lin J, Lin S, Qiao J, Chen ZJ. Association of trophectoderm biopsy with obstetric and perinatal outcomes in singleton pregnancies. Hum Reprod. 2019;34(4):758–65. https://doi.org/10.1093/humrep/dez008.
Palini S, Galluzzi L, De Stefani S, Bianchi M, Wells D, Magnani M, Bulletti C. Genomic DNA in human blastocoele fluid. Reprod Biomed Online. 2013;26(6):603–10. https://doi.org/10.1016/j.rbmo.2013.02.012.
Article CAS PubMed Google Scholar
Stigliani S, Anserini P, Venturini PL, Scaruffi P. Mitochondrial DNA content in embryo culture medium is significantly associated with human embryo fragmentation. Hum Reprod. 2013;28:2652–60.
Xu XP, Gan HY, Li FX, Tian Q, Zhang J, Liang RL, Li M, Yang XX, Wu YS. A method to quantify cell-free fetal DNA fraction in maternal plasma using next generation sequencing: its application in non-invasive prenatal chromosomal aneuploidy detection. PLoS One. 2016;11(1): e0146997. https://doi.org/10.1371/journal.pone.0146997.
Article CAS PubMed PubMed Central Google Scholar
Shamonki MI, Jin H, Haimowitz Z, Liu L. Proof of concept: preimplantation genetic screening without embryo biopsy through analysis of cell-free DNA in spent embryo culture media. Fertil Steril. 2016;106(6):1312–8. https://doi.org/10.1016/j.fertnstert.2016.07.1112.
Article CAS PubMed Google Scholar
Jiao J, Shi B, Sagnelli M, Yang D, Yao Y, Li W, Shao L, Lu S, Li D, Wang X. Minimally invasive preimplantation genetic testing using blastocyst culture medium. Hum Reprod. 2019;34(7):1369–79. https://doi.org/10.1093/humrep/dez075.
Article CAS PubMed Google Scholar
Handayani N, Aubry D, Boediono A, Wiweko B, Sirait B, Sini I, Polim AA, Dwiranti A, Bowolaksono A. The origin and possible mechanism of embryonic cell-free DNA release in spent embryo culture media: a review. J Assist Reprod Genet. 2023;40(6):1231–42. https://doi.org/10.1007/s10815-023-02813-z.
Article PubMed PubMed Central Google Scholar
Griffin DK, Brezina PR, Tobler K, Zhao Y, Silvestri G, McCoy RC, Anchan R, Benner A, Cutting GR, Kearns WG. The human embryonic genome is karyotypically complex, with chromosomally abnormal cells preferentially located away from the developing fetus. Hum Reprod. 2023;38(1):180–8. https://doi.org/10.1093/humrep/deac238.
Article CAS PubMed Google Scholar
Hanson BM, Tao X, Hong KH, Comito CE, Pangasnan R, Seli E, Jalas C, Scott RT. Noninvasive preimplantation genetic testing for aneuploidy exhibits high rates of deoxyribonucleic acid amplification failure and poor correlation with results obtained using trophectoderm biopsy. Fertil Steril. 2021;115(6):1461–70. https://doi.org/10.1016/j.fertnstert.2021.01.028.
Article CAS PubMed Google Scholar
Vera-Rodriguez M, Diez-Juan A, Jimenez-Almazan J, Martinez S, Navarro R, Peinado V, et al. Origin and composition of cell-free DNA in spent medium from human embryo culture during preimplantation development. Hum Reprod. 2018;33:745–56.
Magli MC, Albanese C, Crippa A, Tabanelli C, Ferraretti AP, Gianaroli L. Deoxyribonucleic acid detection in blastocoelic fluid: a new predictor of embryo ploidy and viable pregnancy. Fertil Steril. 2019;111(1):77–85. https://doi.org/10.1016/j.fertnstert.2018.09.016.
Article CAS PubMed Google Scholar
Hammond ER, Shelling AN, Cree LM. Nuclear and mitochondrial DNA in blastocoele fluid and embryo culture medium: evidence and potential clinical use. Hum Reprod. 2016;31(8):1653–61. https://doi.org/10.1093/humrep/dew132.
Article CAS PubMed Google Scholar
Chen J, Jia L, Li T, Guo Y, He S, Zhang Z, Su W, Zhang S, Fang C. Diagnostic efficiency of blastocyst culture medium in noninvasive preimplantation genetic testing. F&S Reports. 2021;2(1):88–94. https://doi.org/10.1016/j.xfre.2020.09.004.
Chen Y, Gao Y, Jia J, Chang L, Liu P, Qiao J, Tang F, Wen L, Huang J. DNA methylome reveals cellular origin of cell-free DNA in spent medium of human preimplantation embryos. J Clin Invest. 2021. https://doi.org/10.1172/JCI146051.
Article PubMed PubMed Central Google Scholar
Huang L, Bogale B, Tang Y, Lu S, Xie XS, Racowsky C. Noninvasive preimplantation genetic testing for aneuploidy in spent medium may be more reliable than trophectoderm biopsy. Proc Natl Acad Sci U S A. 2019;116(28):14105–12. https://doi.org/10.1073/pnas.1907472116.
Article CAS PubMed PubMed Central Google Scholar
Rubio C, Rienzi L, Navarro-Sánchez L, Cimadomo D, García-Pascual CM, Albricci L, Soscia D, Valbuena D, Capalbo A, Ubaldi F, Simón C. Embryonic cell-free DNA versus trophectoderm biopsy for aneuploidy testing: concordance rate and clinical implications. Fertil Steril. 2019;112(3):510–9. https://doi.org/10.1016/j.fertnstert.2019.04.038.
Article CAS PubMed Google Scholar
Yeung QSY, Zhang YX, Chung JPW, Lui WT, Kwok YKY, Gui B, Kong GWS, Cao Y, Li TC, Choy KW. A prospective study of non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) using next-generation sequencing (NGS) on spent culture media (SCM). J Assist Reprod Genet. 2019;36:1609–21.
PubMed PubMed Central Google Scholar
Nakhuda G, Rodriguez S, Tormasi S, Welch C. A pilot study to investigate the clinically predictive values of copy number variations detected by next-generation sequencing of cell-free deoxyribonucleic acid in spent culture media. Fertil Steril. 2024;122(1):42–51. https://doi.org/10.1016/j.fertnstert.2024.02.030.
Article CAS PubMed Google Scholar
Rubio C, Navarro-Sánchez L, García-Pascual CM, Ocali O, Cimadomo D, Venier W, Barroso G, Kopcow L, Bahçeci M, Kulmann MIR, et al. Multicenter prospective study of concordance between embryonic cell-free DNA and trophectoderm biopsies from 1301 human blastocysts. Am J Obstet Gynecol. 2020;223(5):751.e1-751.e13. https://doi.org/10.1016/j.ajog.2020.04.035.
Article CAS PubMed Google Scholar
Navarro-Sánchez L, García-Pascual C, Rubio C, Simón C. Non-invasive preimplantation genetic testing for aneuploidies: an update. Reprod Biomed Online. 2022;44(5):817–28. https://doi.org/10.1016/j.rbmo.2022.01.012.
Article CAS PubMed Google Scholar
Madjunkova S, Kuznyetsov V, Antes R, Motamedi G, Abramov R, Ibarrientos Z, Zaman A, Novoselska A, Kuznyetsova I, Librach CL. Assessment of the impact of blastocyst morphology on the accuracy of non-invasive preimplantation genetic testing (niPGT-A) utilizing culture-conditioned embryo culture medium combined with blastocoel fluid. European Society of Human Reproduction and Embryology (ESHRE). 2019. Vienna
Sakkas D, Navarro-Sánchez L, Ardestani G, Barroso G, Bisioli C, Boynukalin K, Cimadomo D, Frantz N, Kopcow L, Andrade GM, Ozturk B, Rienzi L, Weiser A, Valbuena D, Simón C, Rubio C. The impact of implementing a non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) embryo culture protocol on embryo viability and clinical outcomes. Hum Reprod. 2024;39(9):1952–9. https://doi.org/10.1093/humrep/deae156.
Comments (0)