Validation and clinical study of single-step vitrification combined with single-step warming of human blastocysts

Kuwayama M, Vatja G, Kato O, Leibo S. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online. 2005;11:300–8. https://doi.org/10.1016/S1472-6483(10)60837-1.

Article  PubMed  Google Scholar 

Martinez-Rodero I, Gallardo M, Pisaturo V, Scarica C, Conaghan J, Liebermann J, Cuevas-Sainz I. The development of shorter protocols for vitrification and post-warming dilution of human oocytes and embryos: a narrative review. Reprod BioMed Online. 2025; Articles in Press, 104857. https://doi.org/10.1016/j.rbmo.2025.104857.

Manns JN, Katz S, Whelan III J, Patrick JL, Holt T, Merline AM, Taylor TH. Validation of a new, ultra-fast blastocyst warming technique reduces warming times to 1 min and yields similar survival and re-expansion compared to blastocysts warmed using a standard method. In: ASRM Scientific Congress Expo, Oct 17–20; Baltimore, Maryland, USA. Fertil Steril. 2021;116,Suppl.:e165. https://doi.org/10.1016/j.fertnstert.2021.07.456.

Schiewe MC, Ochoa T, Zeffiro C, Stachecki JJ. Alternative blastocyst vitrification warming dilution approaches: do virtues of the KISS principle apply? In: College of Reproductive Biology (CRB) Symposium, Oct 16–17; on line. Reprod Biomed Online. 2020;41,Suppl. 1:e2-e3. https://doi.org/10.1016/j.rbmo.2020.08.021.

Naert MN, Jiang VS, Dimitriadis I, Souter I, Bormann CL. External validation study of the ultrafast blastocyst warming technique – optimizing efficiency without compromising outcomes. In: ASRM Scientific Congress Expo, Oct 22–26; Anaheim, California, USA. Fertil Steril. 2022;118(4),Suppl.:e128. https://doi.org/10.1016/j.fertnstert.2022.08.379.

Gunst J, Ahlström A. Multicenter preclinical validation of warming procedures for human blastocysts involving a short exposure to a single sucrose solution shows promising survival, re-expansion and continued development. In: 48th Belgian Society for Reproductive Medicine (BSRM) – Scientific Meeting, November 25–26, Brugge, Belgium. 2022;07:28. https://bsrmsite2.wordpress.com/wp-content/uploads/2022/11/48th-bsrm-abstracts.book-v2.pdf. Accessed Nov 21 2022

Emeny-Smith K, Proctor JG, Larman MG, Kirkpatrick S. Ultra-rapid warm: a preclinical comparative analysis of the effect of different sucrose concentrations on blastocyst survival, re-expansion and development post-warm. In: AAB Conference and CRB Symposium, May 9–12, Las Vegas, Nevada, USA. 2023;23. https://www.aab.org/images/CRB/2023/AbstractBooklet2023.pdf

Liebermann J, Wagner Y, Pantos M, Suralski A, Brohammer R, Joris H. Rapid warming of human blastocysts: is 1M sucrose the only choice? In: AAB Conference and CRB Symposium, May 9–12, Las Vegas, Nevada, USA. 2023;26. https://www.aab.org/images/CRB/2023/AbstractBooklet2023.pdf

Abu ali K & Ahlström A. Validation of single step warming for human blastocysts shows successful results are independent of the sucrose concentration used. In: ESHRE 39th Annual meeting, June 25–28, Copenhagen, Denmark. Hum Reprod. 2023;38,Suppl., dead093.542. https://doi.org/10.1093/humrep/dead093.542.

Leibo SP. A one-step method for direct nonsurgical transfer of frozen-thawed bovine embryos. Theriogenology. 1984;21:767–90. https://doi.org/10.1016/0093-691x(84)90022-0.

Article  CAS  PubMed  Google Scholar 

Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil. 1990;89:91–7. https://doi.org/10.1530/jrf.0.0890091.

Article  CAS  PubMed  Google Scholar 

Zozula S, Zwerling M, Whitney J, Anderson RE, Schiewe MC. Microsecure vitrification (µS-VTF): evaluation of thaw dilution intervals for human blastocysts. In: AAB Conference and CRB Symposium, USA. 2012;6–7. https://www.aab.org/images/aab/pdf/12%20abstracts.pdf.

Taylor TH, Manns JN, Katz I, Patrick JL, Whelan J, Katz S. Ultrafast warming protocol demonstrates similar outcomes and significantly decreases embryology workload compared to standard protocols, a randomized control trial with euploid blastocysts. In: ASRM Scientific Congress Expo, Oct 22–26; Anaheim, California, USA. Fertil Steril. 2022;118, Suppl.:e150. https://doi.org/10.1016/j.fertnstert.2022.08.437.

Liebermann J, Hrvojevic K, Hirshfeld-Cytron J, Brohammer R, Wagner Y, Susralski A, et al. Fast and furious: pregnancy outcome with one-step rehydration in the warming protocol for human blastocysts. Reprod BioMed Online. 2023;48:103731. https://doi.org/10.1016/j.rbmo.2023.103731.

Article  PubMed  Google Scholar 

Jiang VS, Cherouveim P, Naert MN, Dimitriadis I, Souter I, Bormann CL. Live birth outcomes following single step blastocyst warming technique - optimizing efficiency without impacting live birth rates. J Assist Reprod Genet. 2024;41:1193–202. https://doi.org/10.1007/s10815-024-03069-x.

Article  PubMed  PubMed Central  Google Scholar 

Lammers J, Reignier A, Loubersac S, Chaillot M, Freour T. Ultra-fast warming procedure of vitrified blastocysts results in maintained embryology and clinical outcomes. Reprod Sci. 2025;32:495–501. https://doi.org/10.1007/s43032-024-01762-x.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lane M, Schoolcraft WB, Gardner DK. Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique. Fertil Steril. 1999;72:1073–8. https://doi.org/10.1016/s0015-0282(99)00418-5.

Article  CAS  PubMed  Google Scholar 

Lane M, Gardner DK. Vitrification of mouse oocytes using a nylon loop. Mol Reprod Dev. 2001;58:342–7. https://doi.org/10.1002/1098-2795(200103)58:3<342::AID-MRD13>3.0.CO;2-X.

Article  CAS  PubMed  Google Scholar 

Mukaida T, Nakamura S, Tomiyama T, Wada S, Kasai M, Takahashi K. Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique. Fertil Steril. 2001;76:618–20. https://doi.org/10.1016/s0015-0282(01)01968-9.

Article  CAS  PubMed  Google Scholar 

Mavrides A, Morroll D. Cryopreservation of bovine oocytes: is cryoloop vitrification the future to preserving the female gamete? Reprod Nutr Dev. 2002;42:73–80. https://doi.org/10.1051/rnd:20020008.

Article  PubMed  Google Scholar 

Reed ML, Lane M, Gardner DK, Jensen NL, Thompson J. Vitrification of human blastocysts using the cryoloop method: successful clinical application and birth of offspring. J Assist Reprod Genet. 2002;19:304–6. https://doi.org/10.1023/a:1015789532736.

Article  PubMed  PubMed Central  Google Scholar 

Son W-Y, Yoon S-H, Yoon H-J, Lee M-S, Lim J-H. Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele. Hum Reprod. 2003;18:137–9. https://doi.org/10.1093/humrep/deg029.

Article  CAS  PubMed  Google Scholar 

Cervera RP, Garcia‐Ximénez F. Vitrification of zona‐free rabbit expanded or hatching blastocysts: a possible model for human blastocysts. Hum Reprod. 2003;18:2151–6. https://doi.org/10.1093/humrep/deg428.

Article  CAS  PubMed  Google Scholar 

Larman MG, Sheehan CB, Gardner DK. Vitrification of mouse pronuclear oocytes with no direct liquid nitrogen contact. Reprod Biomed Online. 2006;12:66–9. https://doi.org/10.1016/S1472-6483(10)60982-0.

Article  PubMed  Google Scholar 

Larman MG, Katz-Jaffe MG, Sheehan CB, Gardner DK. 1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology. Hum Reprod. 2007;22:250–9. https://doi.org/10.1093/humrep/del319.

Article  CAS  PubMed  Google Scholar 

Desai N, Blackmon H, Szeptycki J, Goldfarb J. Cryoloop vitrification of human day 3 cleavage-stage embryos: post-vitrification development, pregnancy outcomes and live births. Reprod Biomed Online. 2007;14:208–13. https://doi.org/10.1016/s1472-6483(10)60789-4.

Article  PubMed  Google Scholar 

Desai N. The new Rapid-I carrier is an effective system for human embryo vitrification at both the blastocyst and the cleavage stage. Reprod Biol Endocrinol. 2013;11:1–9. https://doi.org/10.1186/1477-7827-11-41.

Article  Google Scholar 

Wikland M, Hardarson T, Hillensjö T, Westin C, Westlander G, Wood M, et al. Obstetric outcomes after transfer of vitrified blastocysts. Hum Reprod. 2010;25:1699–707. https://doi.org/10.1093/humrep/deq117.

Article  CAS  PubMed  Google Scholar 

Gook DA, Choo B, Bourne H, Lewis K, Edgar DH. Closed vitrification of human oocytes and blastocysts: outcomes from a series of clinical cases. J Assist Reprod Genet. 2016;33:1247–52. https://doi.org/10.1007/s10815-016-0748-7.

Article  PubMed  PubMed Central  Google Scholar 

Gallardo M, Saenz J, Risco R. Human oocytes and zygotes are ready for ultra-fast vitrification after 2 minutes of exposure to standard CPA solutions. Sci Rep. 2019;9:15986. https://doi.org/10.1038/s41598-019-52014-x.

Article  PubMed  PubMed Central  Google Scholar 

Korkmaz C, Yildiz ÜG, Fidan U, Baykal B, Ceyhan ST, Ağaçayak E. Investigation of transfer results of human embryos that were vitrified and thawed at the cleavage, morula and blastocyst stages. Zygote. 2020;28:191–5. https://doi.org/10.1017/S0967199419000777.

Article  PubMed  Google Scholar 

Schiewe MC. Comparative re-vitrification (RVTF) of human blastocysts aimed at validating ultra-fast vitrification and rapid (one-step) elution (re) approaches. In: ASRM Scientific Congress Expo, Oct 14–18; New Orleans, Louisana, USA. Fertil Steril. 2023;120,Suppl.:e113-e114. https://doi.org/10.1016/j.fertnstert.2023.08.367.

Kaskar K, Sieren K, Brabner V, Huneke B, Vermilyea L, VerMilyea M, Silverberg K. Effect of reducing the vitrification and warming times on blastocyst survival and pregnancy rates. In: ASRM Scientific Congress Expo, Oct 14–18; New Orleans, Louisana, USA. Fertil Steril. 2023;120, Suppl.:e143. https://doi.org/10.1016/j.fertnstert.2023.08.439.

Chaplia O, Ergun Y, Nichols-Burns S, Halicigil C, Basar M. Rapid vs standard: ultrafast warming of vitrified blastocysts does not alter blastocyst competency and stress response. In: ASRM Scientific Congress Expo, Oct 14–18; New Orleans, Louisana, USA. Fertil Steril. 2023;120 Suppl.:e204-e205. https://doi.org/10.1016/j.fertnstert.2023.08.589.

Zhu SE, Kasai M, Otoge H, Sakurai T, Machida T. Cryopreservation of expanded mouse blastocysts by vitrification in ethylene glycol-based solutions. J Reprod Fertil. 1993;98:139–45. https://doi.org/10.1530/jrf.0.0980139.

Article  CAS  PubMed  Google Scholar 

Mahmoudzadeh AR, Van Soom A, Bols P, Ysebaert MT, de kruif A. Optimization of a simple vitrification procedure for bovine embryos produced in vitro: effect of developmental stage, two-step addition of cryoprotectant and sucrose dilution on embryonic survival. J Reprod Fertil. 1995;103:33–9. https://doi.org/10.1530/jrf.0.1030033.

Article  CAS  PubMed  Google Scholar 

Ectors F, Vanderzwalmen P, Dupuis N, Connan D, Grobet L. One-step vitrification of murine embryos challenges current paradigms of cryobiology. Cryobiology. 2018;85:144. https://doi.org/10.1016/j.cryobiol.2018.10.099.

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