Kircher, M. F. et al. A brain tumor molecular imaging strategy using a new triple-modality MRI–photoacoustic–Raman nanoparticle. Nat. Med. 18, 829–834 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yang, J.-M. et al. Simultaneous functional photoacoustic and ultrasonic endoscopy of internal organs in vivo. Nat. Med. 18, 1297–1302 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang, Y. et al. Non-invasive multimodal functional imaging of the intestine with frozen micellar naphthalocyanines. Nat. Nanotechnol. 9, 631–638 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nguyen, V. P. et al. Chain-like gold nanoparticle clusters for multimodal photoacoustic microscopy and optical coherence tomography enhanced molecular imaging. Nat. Commun. 12, 34 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jeng, G.-S. et al. Real-time interleaved spectroscopic photoacoustic and ultrasound (PAUS) scanning with simultaneous fluence compensation and motion correction. Nat. Commun. 12, 716 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ni, R. et al. Multiscale optical and optoacoustic imaging of amyloid-β deposits in mice. Nat. Biomed. Eng. 6, 1031–1044 (2022).

Article  CAS  PubMed  Google Scholar 

Kim, H. et al. Deep laser microscopy using optical clearing by ultrasound-induced gas bubbles. Nat. Photon. 16, 762–768 (2022).

Article  CAS  Google Scholar 

Kang, X. et al. A photo-triggered self-accelerated nanoplatform for multifunctional image-guided combination cancer immunotherapy. Nat. Commun. 14, 5216 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang, Y. et al. In vivo ultrasound-induced luminescence molecular imaging. Nat. Photon. 18, 334–343 (2024).

Article  CAS  Google Scholar 

Ahn, J. et al. Smarter biopsy decisions in thyroid nodules via dual-modal photoacoustic and ultrasound imaging. Sci. Adv. 11, eady6173 (2025).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ruan, H. et al. Deep tissue optical focusing and optogenetic modulation with time-reversed ultrasonically encoded light. Sci. Adv. 3, eaao5520 (2017).

Article  PubMed  PubMed Central  Google Scholar 

Özsoy, Ç., Lafci, B., Reiss, M., Deán-Ben, X. L. & Razansky, D. Real-time assessment of high-intensity focused ultrasound heating and cavitation with hybrid optoacoustic ultrasound imaging. Photoacoustics 31, 100508 (2023).

Article  PubMed  PubMed Central  Google Scholar 

Karakatsani, M. E. et al. Shedding light on ultrasound in action: optical and optoacoustic monitoring of ultrasound brain interventions. Adv. Drug Deliv. Rev. 205, 115177 (2024).

Article  Google Scholar 

Wang, S. et al. Contrast ultrasound-guided photothermal therapy using gold nanoshelled microcapsules in breast cancer. Euro. J. Radiol. 83, 117–122 (2014).

Article  Google Scholar 

Zhang, S., Zhang, E. Z., Beard, P. C., Desjardins, A. E. & Colchester, R. J. Dual-modality fibre optic probe for simultaneous ablation and ultrasound imaging. Commun. Eng. 1, 20 (2022).

Article  Google Scholar 

Cao, Y., Dumani, D. S., Hallam, K. A., Emelianov, S. Y. & Ran, H. Real-time monitoring of NIR-triggered drug release from phase-changeable nanodroplets by photoacoustic/ultrasound imaging. Photoacoustics 30, 100474 (2023).

Article  PubMed  PubMed Central  Google Scholar 

Choi, W. et al. Three-dimensional multistructural quantitative photoacoustic and US imaging of human feet in vivo. Radiology 303, 467–473 (2022).

Article  PubMed  Google Scholar 

Lee, C. et al. Panoramic volumetric clinical handheld photoacoustic and ultrasound imaging. Photoacoustics 31, 100512 (2023).

Article  PubMed  PubMed Central  Google Scholar 

Menozzi, L. et al. Three-dimensional non-invasive brain imaging of ischemic stroke by integrated photoacoustic, ultrasound and angiographic tomography (PAUSAT). Photoacoustics 29, 100444 (2023).

Article  PubMed  Google Scholar 

Kou, S. et al. A portable photoacoustic microscopy and ultrasound system for rectal cancer imaging. Photoacoustics 39, 100640 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Wang, L. V. & Hu, S. Photoacoustic tomography: in vivo imaging from organelles to organs. Science 335, 1458–1462 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Choi, W. et al. Recent advances in contrast-enhanced photoacoustic imaging: overcoming the physical and practical challenges. Chem. Rev. 123, 7379–7419 (2023).

Article  CAS  PubMed  Google Scholar 

Park, J. et al. Clinical translation of photoacoustic imaging. Nat. Rev. Bioeng. 3, 193–212 (2025).

Article  CAS  Google Scholar 

Jiao, S., Xie, Z., Zhang, H. F. & Puliafito, C. A. Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography. Opt. Lett. 34, 2961–2963 (2009).

Article  PubMed  PubMed Central  Google Scholar 

Maslov, K., Stoica, G. & Wang, L. V. In vivo dark-field reflection-mode photoacoustic microscopy. Opt. Lett. 30, 625–627 (2005).

Article  PubMed  Google Scholar 

Zhang, C., Maslov, K. & Wang, L. V. Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo. Opt. Lett. 35, 3195–3197 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Park, E. et al. Unsupervised inter-domain transformation for virtually stained high-resolution mid-infrared photoacoustic microscopy using explainable deep learning. Nat. Commun. 15, 10892 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Hu, S., Maslov, K. & Wang, L. V. Second-generation optical-resolution photoacoustic microscopy with improved sensitivity and speed. Opt. Lett. 36, 1134–1136 (2011).

Article  PubMed  PubMed Central  Google Scholar 

Ahn, J. et al. In vivo photoacoustic monitoring of vasoconstriction induced by acute hyperglycemia. Photoacoustics 30, 100485 (2023).

Article  PubMed  PubMed Central  Google Scholar 

Kim, D. et al. Quantitative volumetric photoacoustic assessment of vasoconstriction by topical corticosteroid application in mice skin. Photoacoustics 40, 100658 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Yao, D.-K., Maslov, K., Shung, K. K., Zhou, Q. & Wang, L. V. In vivo label-free photoacoustic microscopy of cell nuclei by excitation of DNA and RNA. Opt. Lett. 35, 4139–4141 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Park, E., Kim, D., Ha, M., Kim, D. & Kim, C. A comprehensive review of high-performance photoacoustic microscopy systems. Photoacoustics 44, 100739 (2025).

Article  PubMed  PubMed Central  Google Scholar 

Zhang, J. et al. In vivo characterization and analysis of glioblastoma at different stages using multiscale photoacoustic mole