Feng L, Huo Z, Xiong J, Li H. Certification of amyloid-beta (Aβ) certified reference materials by amino acid-based isotope dilution high-performance liquid chromatography mass spectrometry and sulfur-based high-performance liquid chromatography isotope dilution inductively coupled plasma mass spectrometry. Anal Chem. 2020;92(19):13229–37. https://doi.org/10.1021/acs.analchem.0c02381.

Article  PubMed  Google Scholar 

Panteghini M, Braga F, Camara JE, Delatour V, Van Uytfanghe K, Vesper HW, et al. Optimizing available tools for achieving result standardization: value added by Joint Committee on Traceability in Laboratory Medicine (JCTLM). Clin Chem. 2021;67(12):1590–605. https://doi.org/10.1093/clinchem/hvab178.

Article  PubMed  Google Scholar 

Leinenbach A, Pannee J, Dülffer T, Huber A, Bittner T, Andreasson U, et al. Mass spectrometry–based candidate reference measurement procedure for quantification of amyloid-β in cerebrospinal fluid. Clin Chem. 2014;60(7):987–94. https://doi.org/10.1373/clinchem.2013.220392.

Article  PubMed  Google Scholar 

Beasley-Green A, Burris NM, Bunk DM, Phinney KW. Multiplexed LC–MS/MS assay for urine albumin. J Proteome Res. 2014;13(9):3930–9. https://doi.org/10.1021/pr500204c.

Article  PubMed  Google Scholar 

Brzhozovskiy A, Kononikhin A, Bugrova AE, Kovalev GI, Schmit P-O, Kruppa G, et al. The parallel reaction monitoring-parallel accumulation–serial fragmentation (prm-PASEF) approach for multiplexed absolute quantitation of proteins in human plasma. Anal Chem. 2022;94(4):2016–22. https://doi.org/10.1021/acs.analchem.1c03782.

Article  PubMed  Google Scholar 

Kinumi T, Mizuno R, Takatsu A. Quantification of serum C-peptide by isotope-dilution liquid chromatography–tandem mass spectrometry: enhanced detection using chemical modification and immunoaffinity purification. J Chromatogr B. 2014;953:138–42. https://doi.org/10.1016/j.jchromb.2014.02.019.

Article  Google Scholar 

Torkamannejad S, Chang G, Aroge FA, Sun B. Single isotopologue for in-sample calibration and absolute quantitation by LC-MS/MS. J Proteome Res. 2024;23(4):1351–9. https://doi.org/10.1021/acs.jproteome.3c00848.

Article  PubMed  Google Scholar 

Yang S, Tian X, Chen Y, Shen L, Wang J. Isotope-dilution liquid chromatography-tandem mass spectrometry method for serum beta 2-microglobulin quantification. J Chromatogr B. 2022;1211: 123487. https://doi.org/10.1016/j.jchromb.2022.123487.

Article  Google Scholar 

Beasley-Green A, Heckert NA. Estimation of measurement uncertainty for the quantification of protein by ID-LC–MS/MS. Anal Bioanal Chem. 2023;415(16):3265–74. https://doi.org/10.1007/s00216-023-04705-8.

Article  PubMed  PubMed Central  Google Scholar 

Wilffert D, Bischoff R, Van De Merbel NC. Antibody-free workflows for protein quantification by LC–MS/MS. Bioanalysis. 2015;7(6):763–79. https://doi.org/10.4155/bio.14.308.

Article  PubMed  Google Scholar 

Shen Y, Luo X, Li H, Chen Z, Guan Q, Cheng L. Simple and reliable serotonin assay in human serum by LC-MS/MS method coupled with one step protein precipitation for clinical testing in patients with carcinoid tumors. J Chromatogr B. 2020;1158: 122395. https://doi.org/10.1016/j.jchromb.2020.122395.

Article  Google Scholar 

Owusu BY, Pflaum H, Garner R, Foulon N, Laha TJ, Hoofnagle AN. Development and validation of a novel LC-MS/MS assay for C-peptide in human serum. J Mass Spectrom Adv Clinl Lab. 2021;19:1–6. https://doi.org/10.1016/j.jmsacl.2020.12.001.

Article  Google Scholar 

Genet SAAM, Van Den Wildenberg SAH, Broeren MAC, Van Dongen JLJ, Brunsveld L, Scharnhorst V, et al. Quantification of the lung cancer tumor marker CYFRA 21-1 using protein precipitation, immunoaffinity bottom-up LC-MS/MS. Clin Chem Lab Med. 2024;62(4):720–8. https://doi.org/10.1515/cclm-2023-0795.

Article  PubMed  Google Scholar 

Winther B, Nordlund M, Paus E, Reubsaet L, Halvorsen TG. Immuno‐capture as ultimate sample cleanup in LC‐MS/MS determination of the early stage biomarker ProGRP. J Sep Sci. 2009;32(17):2937–43. https://doi.org/10.1002/jssc.200900233.

Article  PubMed  Google Scholar 

Huynh H-H, Bœuf A, Derbez-Morin M, Dupuy A-M, Lalere B, Delatour V, et al. Development of an antibody-free ID-LC-MS method for the quantification of procalcitonin in human serum at sub-microgram per liter level using a peptide-based calibration. Anal Bioanal Chem. 2021;413(19):4707–25. https://doi.org/10.1007/s00216-021-03361-0.

Article  PubMed  Google Scholar 

Sleumer B, Van Faassen M, Vos MJ, Bischoff R, Kema IP, Van De Merbel NC. Selective quantification of the 22-kDa isoform of human growth hormone 1 in serum and plasma by immunocapture and LC–MS/MS. Anal Bioanal Chem. 2022;414(20):6187–200. https://doi.org/10.1007/s00216-022-04188-z.

Article  PubMed  PubMed Central  Google Scholar 

Liu H, Han Y, Wang H, Zhang Y, Liu C, Liang M. A candidate reference measurement procedure for quantification of human insulin in serum based on immunoaffinity extraction and isotope dilution-liquid chromatography-tandem mass spectrometry. Anal Bioanal Chem. 2025;417(16):3731–40. https://doi.org/10.1007/s00216-025-05900-5.

Article  PubMed  Google Scholar 

Mazzarino M, Melis I, Quaresima E, Botrè F. Detection of synthetic analogues of insulin-like growth factor 1 in different biological fluids by liquid chromatography quadrupole time-of-flight mass spectrometry: comparison of different immunoaffinity protocols. Anal Bioanal Chem. 2023;415(24):6117–31. https://doi.org/10.1007/s00216-023-04885-3.

Article  PubMed  Google Scholar 

Halvorsen TG, Reubsaet L. Antibody based affinity capture LC-MS/MS in quantitative determination of proteins in biological matrices. TrAC, Trends Anal Chem. 2017;95:132–9. https://doi.org/10.1016/j.trac.2017.08.009.

Article  Google Scholar 

Van Andel E, Roosjen M, Van Der Zanden S, Lange SC, Weijers D, Smulders MMJ, et al. Highly specific protein identification by immunoprecipitation–mass spectrometry using antifouling microbeads. ACS Appl Mater Interfaces. 2022;14(20):23102–16. https://doi.org/10.1021/acsami.1c22734.

Article  PubMed  PubMed Central  Google Scholar 

Nordlund MS, Warren DJ, Laerdahl JK, Paus E. Studies on multiple forms of proGRP in serum from small cell lung cancer patients. Tumor Biol. 2009;30(5–6):265–75. https://doi.org/10.1159/000258135.

Article  Google Scholar 

Molina R, Filella X, Augé JM. ProGRP: a new biomarker for small cell lung cancer. Clin Biochem. 2004;37(7):505–11. https://doi.org/10.1016/j.clinbiochem.2004.05.007.

Article  PubMed  Google Scholar 

Wojcik E, Kulpa J. Pro-gastrin-releasing peptide (ProGRP) as a biomarker in small-cell lung cancer diagnosis, monitoring and evaluation of treatment response. Lung Cancer: Targets Ther. 2017;8:231–40. https://doi.org/10.2147/lctt.s149516.

Article  Google Scholar 

Torsetnes SB, Levernæs MS, Broughton MN, Paus E, Halvorsen TG, Reubsaet L. Multiplexing determination of small cell lung cancer biomarkers and their isovariants in serum by immunocapture LC-MS/MS. Anal Chem. 2014;86(14):6983–92. https://doi.org/10.1021/ac500986t.

Article  PubMed  Google Scholar 

Newman SS, Hein LA, Adams AM, Soh HT. Theoretical framework and experimental validation of multiplexed analyte quantification using cross-reactive affinity reagents. Anal Chem. 2025;97(35):18896–906. https://doi.org/10.1021/acs.analchem.5c00763.

Article  PubMed  Google Scholar 

Winther B, Reubsaet JL. Determination of the small cell lung cancer associated biomarker pro‐gastrin‐releasing peptide (ProGRP) using LC‐MS. J Sep Sci. 2007;30(2):234–40. https://doi.org/10.1002/jssc.200600319.

Article  PubMed  Google Scholar 

Kumar D, Gautam N, Alnouti Y. Analyte recovery in LC-MS/MS bioanalysis: an old issue revisited. Anal Chim Acta. 2022;1198: 339512. https://doi.org/10.1016/j.aca.2022.339512.

Article  PubMed  PubMed Central  Google Scholar 

Li W, Luo S, Smith HT, Tse FLS. Quantitative determination of BAF312, a S1P-R modulator, in human urine by LC–MS/MS: prevention and recovery of lost analyte due to container surface adsorption. J Chromatogr B. 2010;878(5–6):583–9. https://doi.org/10.1016/j.jchromb.2009.12.031.

Article  Google Scholar 

Ji AJ, Jiang Z, Livson Y, Davis JA, Chu JX, Weng N. Challenges in urine bioanalytical assays: overcoming nonspecific binding. Bioanalysis. 2010;2(9):1573–86. https://doi.org/10.4155/bio.10.114.

Article  PubMed  Google Scholar 

Yoshimura T, Fujita K, Kinukawa H, Matsuoka Y, Patil RD, Beligere GS, et al. Development and analytical performance evaluation of an automated chemiluminescent immunoassay for pro-gastrin releasing peptide (ProGRP). Clin Chem Lab Med. 2009;47(12):1557–63. https://doi.org/10.1515/cclm.2009.333.

Article  PubMed  Google Scholar 

Dong A, Zhang J, Chen X, Ren X, Zhang X. Diagnostic value of ProGRP for small cell lung cancer in different stages. J Thorac Dis. 2019;11(4):1182–9.https://doi.org/10.21037/jtd.2019.04.29

Yuan F-F, Wang P, Han X-J, Qin T-T, Lu X, Bai H-J. Efficient and rapid digestion of proteins with a dual-enzyme microreactor featuring 3-D pores formed by dopamine/polyethyleneimine/acrylamide-coated KIT-6 molecular sieve. Sci Rep. 2024;14(1): 15667. https://doi.org/10.1038/s41598-024-65045-w.

Article