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Biochemical characterization of heterologously expressed Thiomonas delicata arsenite oxidase subunits (AioA and AioB)
Arsenite oxidase (Aio) plays a central role in the microbial oxidation of As(III) to the generally less toxic and more eas...
The potential and innovative applications of CRISPR gene editing technology in enzyme gene development
This review innovatively proposes for the first time that CRISPR gene editing technology can be used for site specific fus...
Naringinase: A comprehensive review on its characteristics, production, and biotechnological applications
Naringinase (E.C. 3.2.1.40) is an enzyme complex that's recently garnered significant attention due to its enzymatic activ...
Cellulose biosynthesis in nature and In Vitro: mechanisms and challenges
Cellulose is a polysaccharide predominantly found in the extracellular matrices of plants, algae and bacteria [1], with ur...
Enzymatic synthesis of sucrose esters: Advances and challenges in high-efficiency and regioselective catalysis
Sucrose esters (SEs), commonly referred to as fatty acid sucrose esters, are amphiphilic compounds synthesized via the est...
Levansucrase from Gluconacetobacter diazotrophicus. Key residues involved in levan synthesis
The polysaccharide levan is a homopolymer that reaches 104 fructose units and is structured in β-(2→6) linked main chains,...
Enhanced activity and stability of thermostable endoglucanase Dictyoglomus turgidum DtCelA with multiple strategies
Lignocellulosic biomass has been generally recognized to be an abundant and renewable feedstock for sustainable energy pro...
Integrated bio-nano remediation of produced water using Alcanivorax borkumensis and GQDs-modified ZnO/La₂O₃ nanocomposites
Environmental pollution caused by produced water from petroleum extraction remains one of the most persistent and complex ...
Immobilization of α-glucosidase on polystyrene plates: A practical application to α-amylase detection
α-Glucosidase is in high demand for applications involving the digestion of α-configured glycoside and saccharide substrat...
Dual antioxidant and antibacterial potential of β-cryptoxanthin and a novel biosurfactant from Kocuria marina DAGII
The worldwide predominance of microbial infections and oxidative stress-related disorders has caused a significant challen...
The underlying thermal inactivation mechanism and corresponding effective stabilization strategy for Rhizopus oryzae lipase
Lipases are a class of serine hydrolases that catalyze the hydrolysis of triglycerides as well as a variety of other react...
Structure-guided engineering of CYP82D213 for enhanced triptonide biosynthesis
Triptonide (TN) is a diterpenoid natural product with significant antitumor [1], [2], immunosuppressive [3], [4], and anti...
Structural, quantitative, and functional characterization of N-glycans in porcine pancreatin extract, lipase, and α-amylase
Porcine pancreatin extract (PPE, EC 232-468-9) is widely used as a pancreatic enzyme replacement therapy (PERT) to compens...
Nicotinic acid production from 3-methylpyridine by E. coli whole-cell biocatalyst
Niacin (vitamin B3), an essential nutrient and precursor in the biosynthesis of NAD(P), exists primarily in two forms: nic...
Soluble and pocket engineering of D-amino acid oxidase and its application in the biotransformation of L-glufosinate
Glufosinate (2-amino-4-methyl-phosphino-butyric acid, PPT) is a non-selective herbicide, with advantages, including enviro...
Influence of a rumen ciliate-derived xylanase on the gut microbiota composition: A potential enzyme for prebiotic applications
A xylanase derived from rumen ciliates designated as XynC, was successfully expressed and purified. The enzyme's catalytic...
Characterization of a GH10 family thermophilic, alkali- and salt-tolerant xylanase from Xinjiang salt lake
Hemicellulose is the second most prevalent polysaccharide in nature, following cellulose, and accounts for nearly one-thir...
Semi-rational engineering of 3-Ketosteroid-Δ1-dehydrogenase boosts catalytic efficiency and robustness for steroid bioconversion
Steroidal compounds, characterized by a cyclopentanoperhydrophenanthrene backbone, are natural products whose types and po...
Expanding fluorescence imaging tools in Staphylococcus aureus: Optimized expression of red fluorescent proteins
Fluorescent proteins (FPs) are widely used as molecular imaging tools for visualizing protein localization, gene expressio...
Arazyme prevents skin aging through regulation of matrix metalloproteinase and collagen synthesis
As the global aging population continues to grow, there is an increasing societal and commercial demand for effective stra...
Efficient isoprimeverose production using an enzyme cocktail from engineered Aspergillus oryzae and yeast-assisted purification
A rare group of mono- or disaccharides and their derivatives that are found in small quantities in nature have garnered si...
The effect of the cspA gene on growth development and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona
Butenyl-spinosyn, a type I polyketide macrolide compound [1], [2], is derived from spinosyns produced through the aerobic ...