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An optimized C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins
An optimized C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins
Biomolecular dynamics in the microsecond-to-millisecond (µs–ms) timescale are linked to various biological func...
Analyzing sub-millisecond timescale protein dynamics using eCPMG experiments
Analyzing sub-millisecond timescale protein dynamics using eCPMG experiments
Cellular functions require biomolecules to transition among various conformational sub-states in the energy landscape. A m...
Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning
Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning
The use of 1H detection, made possible by very fast magic-angle spinning (MAS), has revolutionized the field of biomolecul...
Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in H-detected protein solid-state NMR
Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in H-detected protein solid-state NMR
We present a novel approach for side-chain-selective deuteration of proteins to improve 1Hα spectral resolution and t...
A fast and efficient strategy for the NMR assignment of Fab methyl groups
A fast and efficient strategy for the NMR assignment of Fab methyl groups
Owing to their high specificity and therapeutic effectiveness, monoclonal antibodies (mAbs) have rapidly become one of the...
M9 medium composition alters metabolism during recombinant expression
M9 medium composition alters metabolism during recombinant expression
M9 minimal media and its enhanced variants (M9 + and M9++) are widely used for recombinant protein expression ...
Single spin exact gradients for the optimization of complex pulses and pulse sequences
Single spin exact gradients for the optimization of complex pulses and pulse sequences
The efficient computer optimization of magnetic resonance pulses and pulse sequences involves the calculation of a problem...
F-NMR spectroscopy of fluorinated isoleucine analogues in a protein
F-NMR spectroscopy of fluorinated isoleucine analogues in a protein
The R3H domain of the human protein Sµbp-2 was produced with 5-fluoro-L-isoleucine (FIle) and 5,5-difluoro-L-isoleuci...
Web server DDfit: a new scheme to process PFG NMR diffusion data with improved precision
Web server DDfit: a new scheme to process PFG NMR diffusion data with improved precision
In this communication we describe a new scheme to process the data from stimulated echo protein diffusion experiments. For...
Conformation-selective detection of residues in solid proteins under magic-angle-spinning
Conformation-selective detection of residues in solid proteins under magic-angle-spinning
We demonstrate here a pulse sequence based on rotational-echo double resonance (REDOR) that can help distinguish resonance...
Toward site-specific characterization of structural perturbations on glycosylated Fc using NMR at natural abundance
Toward site-specific characterization of structural perturbations on glycosylated Fc using NMR at natural abundance
Monoclonal antibodies (mAbs) are leading therapeutic agents due to their high specificity and limited side effects. Ensuri...
Incorporating the BEST methodology in experiments for measuring paramagnetic relaxation enhancements
Incorporating the BEST methodology in experiments for measuring paramagnetic relaxation enhancements
Band-selective excitation short-transient (BEST) sequences are widely used for protein NMR experiments that start with ami...
, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations
, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations
Isotopic enrichment of pharmacologically relevant protein targets is crucial for structural studies by nuclear magnetic re...
Optimus peak: automatic peak information extraction from 2D and 3D solution and solid state spectra using evolutionary algorithms
Optimus peak: automatic peak information extraction from 2D and 3D solution and solid state spectra using evolutionary algorithms
Peak information extraction is an important step for all types of protein NMR experiments, from structure determination to...
Super-Resolution solid-state NMR Spectroscopy
Super-Resolution solid-state NMR Spectroscopy
Solid-state NMR spectroscopy is often limited by low spectral resolution, a problem typically addressed using fast magic-a...
AMIGO - Guided assignment of C-methyl labelled proteins
AMIGO - Guided assignment of C-methyl labelled proteins
Over the last 20 years, the number of large proteins accessible to protein-NMR analysis has increased significantly due to...
Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization
Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization
Monoclonal antibodies (mAbs) are essential therapeutic agents whose efficacy depends critically on the integrity of their ...
Solution NMR study of the titin I-band IgI domain I82 shows unusual conformational dynamics
Solution NMR study of the titin I-band IgI domain I82 shows unusual conformational dynamics
β-sandwich domains of the intermediate immunoglobulin fold (IgI) are key building blocks of many large, multidomain p...
Sedimentation of large, soluble proteins up to 140 kDa for H-detected MAS NMR and C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for H-detected MAS NMR and C DNP NMR – practical aspects
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa wherea...
Quantitative analysis of the slow exchange process by F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-F probe in nucleic acids
Quantitative analysis of the slow exchange process by F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-F probe in nucleic acids
Solution NMR spectroscopy is a particularly powerful technique for characterizing the functional dynamics of biomolecules,...
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, mater...
Optimising in-cell NMR acquisition for nucleic acids
Optimising in-cell NMR acquisition for nucleic acids
Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology and...
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Side chain isotope labelling is a powerful tool to study protein structure and interactions by NMR spectroscopy. 1H,13C la...
Pitfalls in measurements of relaxation rates of protein backbone N nuclei
Pitfalls in measurements of relaxation rates of protein backbone N nuclei
The dynamics of the backbone and side-chains of protein are routinely studied by interpreting experimentally determined 15...
F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
The recent application of 19F NMR in the study of biomolecular structure and dynamics has made it a potentially attractive...
Using temperature coefficients to support resonance assignment of intrinsically disordered proteins
Using temperature coefficients to support resonance assignment of intrinsically disordered proteins
The resonance assignment of large intrinsically disordered proteins (IDPs) is difficult due to the low dispersion of chemi...
Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins
Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins
Inclusion of residual dipolar couplings (RDCs) during the early rounds of protein structure determination requires use of ...
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements ...
N-detected TROSY for H-N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality
N-detected TROSY for H-N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality
Intrinsically disordered proteins and protein regions are central to many biological processes but difficult to characteri...
Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding
Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding
The NMR signals from protein sidechains are rich in information about intra- and inter-molecular interactions, but their d...
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